博尔登和的生产来自植物通过一级联生物转化
Vyacheslav V Kollerov1, Tatiana A Timakova1, Andrei A Shutov1
1Federal Research Center, Pushchino Center for Biological Research of Russian Academy of Sciences, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Prospekt Nauki, 5, 142290 Pushchino, Moscow Region, Russia.
Journal of fungi (Basel, Switzerland)
|December 27, 2024
概括
这项研究开发了一种绿色生物转化过程,用于从植物中生产和博尔登. 使用特定微生物菌株的新级联方法有效地将植物醇转化为这些有价值的类固醇.
科学领域:
- 生物技术和工业微生物学
- 类固醇的生物转化
- 酵素工程是什么意思 酵素工程
背景情况:
- (TS) 和博尔登 (BD) 是重要的类固醇,在医学和兽医科学中具有广泛的应用.
- 目前的生产方法通常涉及复杂的化学合成或低效的酶路径.
- 从易于获得的前体如植物 (PS) 来生产绿色和可持续的类固醇是非常可取的.
研究的目的:
- 从植物 (PS) 开发一种新的级联生物转化工艺,用于从植物 (PS) 生产 (TS) 和博尔登 (BD).
- 为了研究 *Curvularia* sp. 的酶能力. VKM F-3040用于转化类固醇.
- 探索通过生物转化合成7-基化类固醇的潜力.
主要方法:
- 使用的 *Curvularia* sp. 使用过的 *Curvularia* sp. VKM F-3040用于将安德罗沙二 (ADD) 转化为博尔登 (BD).
- 开发了两阶段的级联生物转化:第一阶段涉及*Mycolicibacterium neoaurum*将PS转化为androstenedione (AD) 或ADD;第二阶段使用*Curvularia* sp. 为了减少AD/ADD到TS/BD.
- 采用转录组分析来识别编码17β-hydroxysteroid脱酶 (17β-HSDs) 和P450 7-monooxygenase的候选基因. 研究了循环赫西米德 (CHX) 对基化反应的影响.
主要成果:
- * 曲杆菌 * sp. 这种病. 有效地将ADD转换为BD (收益率高达97%).
- 开发的级联生物转化实现了超过90%的效率,将AD/ADD从PS转化为TS/BD.
- 鉴定了可诱导的P450cur 7-单氧基酶活性,导致显著的7α-基 (7α-OH-TS) 产量 (高达83%) 从AD,由环合胺抑制.
结论:
- 这种 *Curvularia* sp. 这种菌株对类固醇合成具有显著的生物技术潜力.
- 级联生物转化为生产,博尔登和新型7基化类固醇提供了一个有前途的绿色途径.
- 这些发现为可持续的工业合成有价值的类固醇化合物打开了前景.
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