功能性表征FgAsp,一个基因编码酸蛋白酶在Fusarium graminearum
Ping Li1, Zhizhen Fu1, Mengru Wang1
1Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, College of Agriculture, Yangtze University, Jingzhou 434025, China.
酸蛋白酶FgAsp对于Fusarium graminearum的生长,发育和病原性至关重要. 淘汰FgAsp会减少真菌生长,子产生和脱氧烯积累,影响小麦头部病.
科学领域:
- 分子生物学分子生物学
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
背景情况:
- 酸蛋白酶 (APs) 是对真菌生长和病变产生至关重要的酶.
- 草菌 (Fusarium graminearum) 在小麦中引起草菌头部炎 (FHB),这是一个重要的农业疾病.
- 在F. graminearum中APs的特定作用仍然在很大程度上未被描述.
研究的目的:
- 为了研究F. graminearum.中的阿斯巴达蛋白酶基因FGSG_09558 (FgAsp) 的功能.
- 阐明FgAsp在真菌生长,发育和病原性中的作用.
- 确定FgAsp在脱氧尼瓦伦醇 (DON) 生产和应激反应中的参与.
主要方法:
- 在F. graminearum.中对FGSG_09558的基因淘汰和补充.
- 野生型和突变菌株关于生长和分泌的表型分析.
- 在玉米胡须,小麦芽盖和小麦耳上进行病毒性测试.
- 测量脱氧尼瓦伦醇 (DON) 积累的量化.
- 评估糖的利用和对过氧化压力的反应.
主要成果:
- FgAsp淘汰突变体表现出显著减少细胞生长,无性和性子生产.
- 突变者在各种植物组织上显示出毒性降低和DON积累.
- FgAsp影响了糖的利用,尤其是阿拉比诺糖.
- 突变者对过氧化更敏感,表明应激反应受损.
结论:
- FgAsp对于F. graminearum的生长,发育和病原性至关重要.
- 部分通过其对DON毒素积累的影响,FgAsp调节病原性.
- 在真菌对环境压力的反应中,FgAsp起着重要作用.
- 在F. graminearum. 中,FgAsp是多个重要过程的关键调节者.
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