从随机扰动到精确准:对研究Monascus物种基因功能方法的全面审查
Yunxia Gong1,2, Shengfa Li2, Deqing Zhao2
1College of Food Science and Technology, Wuhan Business University, Wuhan 430056, China.
Journal of fungi (Basel, Switzerland)
|December 27, 2024
概括
这篇评论探讨了Monascus真菌的基因操纵工具,重点关注Agrobacterium tumefaciens和基因编辑. 这些方法对于了解Monascus代谢和改善生物技术应用至关重要.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 分子遗传学 分子遗传学
背景情况:
- 蒙纳斯菌产生有价值的生物活性化合物,如颜料和Monacolin K,具有重要的生物技术潜力.
- 了解Monascus代谢的遗传基础是优化发酵和产品产量的关键.
- 对Monascus spp.的遗传知识有限. 阻碍了分子层面对它们的生理学和生物化学的理解.
研究的目的:
- 审查和总结适用于Monascus物种的基因操纵工具.
- 要突出Agrobacterium tumefaciens介导的转化和核酶引导的基因编辑技术.
- 为研究人员选择工具来研究Monascus中的基因功能提供参考资料.
主要方法:
- 关于蒙纳斯科斯基基因操纵现有文献的综述.
- 重点是Agrobacterium tumefaciens介导的转变.
- 专注于核酶引导的基因编辑技术.
主要成果:
- 对Monascus.的各种遗传操纵工具的识别和摘要.
- 详细讨论Agrobacterium tumefaciens和基因编辑作为有效的方法.
- 为工具选择提供全面的参考.
结论:
- 基因操纵工具对于推动Monascus研究至关重要.
- 阿格罗细菌tumefaciens和基因编辑为Monascus的功能基因组学提供了强大的方法.
- 这一审查有助于对Monascus基因功能进行研究,以促进生物技术的进步.
更多相关视频
相关概念视频
02:33Combinatorial Gene Control
8.2K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.2K
01:09Epistasis Analysis
4.8K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
4.8K
02:02Genomics
35.2K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
35.2K