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使用微生物源追踪 (MST) 和环境DNA (eDNA) 分析在菲律宾西尔韦河 (Silway River) 的便污染的评估

Lonny Mar Opog¹, Joan Cecilia Casila², Rubenito Lampayan²

¹Agricultural and Biosystems Engineering Department, College of Agriculture, Mindanao State University, General Santos City 9500, Philippines.

Journal of xenobiotics

|December 27, 2024

概括

的便细菌污染了Silway河,影响了水质和健康. 低流速和溶解氧 (DO) 加剧了这种污染,对生态系统和当地社区构成风险.

科学领域:

环境微生物学环境微生物学
评估水质水质的评估.
分子生物学分子生物学

背景情况:

由于废物管理不充分,Silway河历来超过了安全的便大肠杆菌水平.
桑托斯将军城市的农业 (家禽,牲畜) 的突出地位有助于污染物排放.
之前的研究指出家禽养殖的影响,但这是第一个使用qPCR检测家禽特异性便大肠杆菌.

研究的目的:

在Silway河中检测和量化家禽便污染.
调查流速和物理化学参数对便污染的影响.
使用微生物源追踪 (MST) 和环境DNA (eDNA) 进行精确的污染分析.

主要方法:

部署定量聚合酶连锁反应 (qPCR) 用于敏感检测便标记物.
使用基因标记物Ckmito和ND5-CD用于微生物源追踪 (MST).
应用环境DNA (eDNA) 分析,用于全面的污染评估.
进行多重回归分析,以将水质参数与污染水平相关联.

主要成果:

在Silway河的所有采样站检测到便细菌.
便大肠杆菌扩散的最佳条件包括正常温度,pH值和高总悬浮固体 (TSS).
低流速和低溶解氧 (DO) 与便污染增加有显著的相关性,这是ND5-CD标志物的较低周期值 (Ct) 值所表明的.

结论:

家禽养殖对Silway河的便污染作出了重大贡献.
水质参数,特别是低流速和低DO,是影响污染水平的关键因素.
发现的便污染对水资源,公共卫生,水生生态系统和当地工业构成重大风险.

关键词:

西尔韦河 (Silway River) 是一个河流.的便污染的便污染电子DNA分析电子DNA分析便污染污染便污染流速流速流速流速微生物源追踪的追踪方法水的质量水的质量.

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