非向代谢学分析揭示了在乳清发酵过程中与Kluyveromyces marxianus进行代谢资料的变化
Yansong Gao1,2, Lei Gao1,2, You Kang1,2
1Institute of Agro-Food Technology, Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, China.
Metabolites
|December 27, 2024
概括
乳清发酵由Kluyveromyces marxianus KM812丰富代谢物,增强营养和功能性质. 这一过程产生生物活性小分子,支持新型功能性食品的开发.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
背景情况:
- 乳清发酵可以产生具有免疫调节,代谢综合征调节和抗氧化作用的生物活性化合物.
- 这个过程为开发具有促进健康属性的新型功能性食品提供了潜力.
研究的目的:
- 为了研究由Kluyveromyces marxianus KM812.12发酵的乳清中的代谢物概况变化.
- 分析发酵时间对代谢物丰富的影响,并确定关键的代谢途径.
主要方法:
- 使用液体染色学质谱法 (LC-MS) 的非向代谢学.
- 在不同时间点对由Kluyveromyces marxianus菌株KM812发酵的乳清进行分析.
主要成果:
- 确定了151种不同的代谢物,主要是氨基酸,,脂肪酸和碳水化合物.
- 代谢物含量逐渐增加,在48小时达到峰值,出现显著的折叠变化.
- 凯格分析将23种代谢物与9种途径联系起来,主要是氨基酸和脂质代谢.
结论:
- Kluyveromyces marxianus KM812有效地将乳清大分子降解为有益的小分子,如L-单氨酸和α-烯酸.
- 发酵改变了乳清的营养和功能性质,为功能性食品开发提供了基础.
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