暴露于二氧化产品会改变人类细胞中的DNA甲基化
Carlos Wells1, Marta Pogribna1, Arjun Sharmah2
1Division of Biochemical Toxicity, FDA/National Center for Toxicological Research, Jefferson, AR 72079, USA.
Nanomaterials (Basel, Switzerland)
|December 27, 2024
概括
二氧化 (TiO2) 暴露会影响人类细胞中的DNA甲基化,影响全球甲基化和特定的基因促进体. 这种表观毒性强调了需要进一步评估消费品中TiO2的风险.
科学领域:
- 环境健康 环境健康
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 毒理学 毒理学 毒理学
背景情况:
- 二氧化 (TiO2) 在消费品中广泛使用,由于报告的毒性,这引发了安全问题.
- 表观遗传机制,如DNA甲基化,在生物对环境暴露的反应中起着至关重要的作用.
研究的目的:
- 调查二氧化 (TiO2) 暴露对人类细胞系中与暴露相关的DNA甲基化发生的表观毒性影响.
- 评估TiO2对全球DNA甲基化和特定基因促进物甲基化的影响.
主要方法:
- 在TiO2暴露研究中利用了Caco-2 (结肠直肠) 和HepG2 (肝脏) 人类细胞系.
- 通过基于ELISA的免疫化学分析评估了全球DNA甲基化.
- 通过EpiTect Methyl II签名PCR阵列评估基因促进物甲基化,并使用qRT-PCR量化基因表达.
主要成果:
- 在TiO2暴露后观察到Caco-2和HepG2细胞的全球DNA甲基化下降.
- 在细胞系中发现了7个基因 (包括TP53和BNIP3) 的促进体的甲基化变化.
- 检测到DNA甲基转移酶,MBD2和UHRF1的异常表达,与甲基化变化相关.
结论:
- 暴露于TiO2显然会影响人类结直肠和肝细胞中的DNA甲基化模式.
- 这些发现支持表观遗传学在调解TiO2的生物效应和毒性方面的作用.
- 表观遗传学评估对于全面评估TiO2对人类健康的风险至关重要.
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