来自Amphitrite ornata的多功能催化血红蛋白:隔离,分类识别,蛋白质提取,净化和表征的协议
Anna L Husted1, Victoria R Sutton1, Lauren A Presnar1
1Department of Natural Sciences, University of South Carolina Beaufort, 1 University Boulevard, Bluffton, SC 29909, USA.
Methods and protocols
|December 27, 2024
概括
研究人员开发了一种DNA条形编码方法,可以轻松识别多基虫Amphitrite ornata. 这有助于在海洋无脊椎动物中发现具有独特催化能力的新型脱氧化酶 (AoDHP).
科学领域:
- 生物化学 生化学
- 海洋生物学 海洋生物学
- 遗传学 是一个遗传学.
背景情况:
- 甲基聚合物甲酸 (Amphitrite ornata) 具有具有氧运输和基质氧化活动的多功能脱氧化酶 (AoDHP).
- 鉴定来自宏观内动物的新型dehaloperoxidases是具有挑战性的,因为分类学识别困难.
研究的目的:
- 开发一种简化的方法,用于准确地分类识别Amphitrite ornata.
- 促进在海洋无脊椎动物中发现新的脱氧化酶候选物.
主要方法:
- 使用线粒体细胞染色体c氧化酶子单元I基因进行DNA条形编码,用于安菲特里特形物识别.
- 从整个虫标本中提取和净化AoDHP.
- 在大肠杆菌中过度表达复合的AoDHP异酶A,以进行活性比较.
主要成果:
- 建立了一种简化DNA条形码识别方法,适合非专家使用.
- 过氧化酶活性测定显示,本源和重组AoDHP迈凯利斯-门参数之间存在强烈的相关性.
- 这项研究成功地从Amphitrite ornata中净化和表征了AoDHP.
结论:
- 描述的方法简化了Amphitrite ornata的分类学识别,有助于脱氧化酶的发现.
- 预计这种方法将加速从其他海洋无脊椎动物中识别新型dehaloperoxidases.
相关概念视频
Hemoglobin
3.0K
Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well.
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
3.0K
Oxygen Transport in the Blood
2.2K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
2.2K
Cooperative Allosteric Transitions
7.8K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
7.8K
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation
3.2K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.2K
Electron Transport Chain: Complex III and IV
6.7K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
6.7K
Gene Families
8.7K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
8.7K


