在Aspergillus flavus隔离物中可奇酸生产和玉米感染的变化表明作为病毒性因素的潜在作用
Rebecca R Sweany1, Matthew K Gilbert1, Carol H Carter-Wientjes1
1Food and Feed Safety Research Unit, Southern Regional Research Center, US Department of Agriculture, New Orleans, LA 70124, USA.
Toxins
|December 27, 2024
概括
酸是一种由Aspergillus flavus产生的抗氧化剂,可能会增强真菌毒性和玉米感染. 非aflatoxigenic菌株产生更多的酸,表明其在生物控制策略中的潜在作用.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 生物化学 生物化学
背景情况:
- 酸是来自Aspergillus物种的二次代谢物,具有抗氧化和合性质.
- 它的生态作用和对真菌毒性的贡献尚不清楚.
- 之前的研究表明,在与亚毒性菌株共同培养期间,非亚毒性阿斯伯吉洛斯亚菌株的酸基因表达增加,与亚毒性菌株的亚毒素产量减少相关.
研究的目的:
- 为了研究来自路易斯安那州的Aspergillus flavus分离物中的 kojic 酸生产.
- 为了比较酸生产与玉米核感染水平.
- 探索酸生产,硬化组织大小和传染性之间的关系.
主要方法:
- 在22种来自路易斯安那州的Aspergillus flavus分离物和4种常见菌株中量化了酸产量.
- 在液体介质和玉米核上评估了产量.
- 玉米核感染是通过滴滴数字PCR (ddPCR) 测量玉米β管蛋白DNA含量.
主要成果:
- 通过Aspergillus flavus分离物产生更多的酸,与较高水平的玉米核感染有关.
- 隔离物产生的酸明显高于阿弗拉托克辛和环酸.
- 具有中型和大型硬化菌的Aspergillus flavus菌株,经常从玉米中分离出来,产生更多的酸,比小型硬化菌菌菌株更具传染性.
结论:
- 酸可能起到毒性因子的作用,增强某些Aspergillus flavus菌株的病原性成功.
- 来自路易斯安那州的非亚弗拉托基因分离物表现出最高的酸产量,这表明生物控制应用的潜力.
- 需要进一步的研究,以阐明酸在感染期间的氧化还原环境中的作用及其对亚毒素减少的影响.
关键词:
非洲毒素 (aflatoxin) 是一种毒素.生物控制 生物控制ddPCRR 在线播放菌类毒素 (mycotoxins) 是一种有毒的物质.病原性是一种病原性.植物真菌相互作用二次新陈代谢二次新陈代谢更多相关视频
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