咖啡酸乙醇对通过调节线粒体和内分泌网膜对胚胎发育的影响
Chu-Man Huang1, Hui-Mei Huang1, Ying-Hua Li1
1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529000, China.
Veterinary sciences
|December 27, 2024
概括
咖啡酸乙烯 (CAPE) 通过改善线粒体功能和减少氧化和内分泌网膜压力来增强猪胚胎发育. 这种天然化合物促进了胚胎细胞的形成和增殖,表明其在辅助生殖技术中的潜力.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 生物化学 生化学
背景情况:
- 蜂含有咖啡酸乙烯 (CAPE),以抗氧化和抗瘤特性而闻名.
- 胚胎质量对于成功的体外受精和辅助生殖技术至关重要.
- 氧化和内质网膜应激会对胚胎发育产生负面影响.
研究的目的:
- 在体外研究CAPE对猪胚胎发育的影响.
- 阐明CAPE影响线粒体和内质网的机制.
- 评估CAPE对猪胚胎中氧化应激,亡和自的影响.
主要方法:
- 在体外培养猪胚胎时,CAPE的度各不相同.
- 评估了发育能力,胚胎细胞形成率和增殖.
- 测量了线粒体膜潜力,ATP含量和关键蛋白质 (PGC1α/β,Nrf2,GRP78) 的表达.
- 测量了活性氧物种 (ROS) 和谷氨水平.
- 评估了亡和自的标志物.
主要成果:
- 1 nM CAPE显著改善了猪胚胎质量,胚芽细胞的形成和繁殖.
- 通过增加膜潜力,PGC1α/β表达和ATP水平,CAPE增强了线粒体功能.
- 通过减少ROS和增加谷氨,CAPE减轻了氧化应激.
- 通过对Nrf2进行上调和对GRP78.8进行下调,CAPE减少了内等质网膜应激.
- 在培养胚胎中,CAPE降低了亡和自.
结论:
- CAPE提高了体外生产的猪胚胎的发育能力.
- CAPE增强了线粒体功能和生物发生.
- CAPE可以减轻氧化和内质网膜压力,从而改善胚胎质量.
- 在改善猪的辅助生殖技术方面,Cape显示出前景.
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