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精子功能状态:牛精子受精潜力的多参数评估

Timea Sarah Odinius^1,2, Mathias Siuda^1,2, Matthias Lautner³

¹Clinic of Reproductive Medicine, Department for Farm Animals, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland.

Veterinary sciences

|December 27, 2024

概括

评估公牛精子的DNA完整性,而不仅仅是生存能力,显著改善了冷保存精子批次的生育预测. 高DNA碎片化表明较低的非返回率,这对于人工授精成功至关重要.

科学领域:

生殖生物学生殖生物学
动物科学动物科学
精子冷保存精子冷保存

背景情况:

精子活力测试是冷保存的公牛精子质量控制的标准,但不能可靠地预测生育能力.
在使用冷保存精液的人工授精计划中,生育潜力至关重要.

研究的目的:

评估将公牛精子生存能力与DNA完整性或功能精子状态相结合的生育预测价值.
确定这些综合指标是否可以准确预测冷保存精子批次的非回报率 (NRR).

主要方法:

流细胞计被用来评估精子活力,DNA碎片化指数 (%DFI),细胞内和线粒体功能.
使用了精子染色素结构检测和五色染色面板.
线性混合效应模型和条件推理树分析了来自791和733个冷保存批次的NRR记录的数据.

主要成果:

具有DNA碎片化指数 (DFI) ≤6.86%的批量与较高的非返回率 (> 60%) 有关.
DFI值>6.86%与较低的非回报率相关 (55-60%或更低).
将解后的活力与活性的精子的功能状态相结合并不能可靠地预测NRR.

结论:

纳入DNA完整性评估,特别是DFI,大大提高了公牛精子生育率的预后准确性.
在冷保存的公牛精子中,DNA完整性是生育潜力的更可靠的指标,而不是精子活力或功能状态.

关键词:

雄牛精子的精子是什么?生育能力生育能力生育能力生育能力流动细胞计量是流动细胞计量的方法.质量控制质量控制质量控制

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