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Updated: May 8, 2025

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光在现场杂交协议可视化Oomycetes在体外和在植物中的

Glen Groben^1,2, Catalina Salgado-Salazar³, Jo Anne Crouch⁴

¹United States Department of Agriculture, Agricultural Research Service, Foreign Disease/Weed Science Research Unit, Frederick, MD, USA.

Methods in molecular biology (Clifton, N.J.)

|December 27, 2024

概括

这项研究介绍了一种新的光现场杂交 (FISH) 协议,用于在没有培养的情况下可视化菌体结构. 该方法有效地检测环境和植物样本中的各种虫生命阶段.

科学领域:

微生物学微生物学
植物病理学植物病理学
分子生物学分子生物学

背景情况:

菌体是重要的植物病原体,需要准确的识别.
传统方法通常依赖于培养,这可能耗时,可能并不总是成功.
在现场杂交技术提供微生物的直接可视化.

研究的目的:

开发和描述一个简化的光现场混合 (FISH) 协议.
为了能够直接可视化各种菌体结构 (菌体,菌体,胞体).
在殖民植物组织中应用检测oomycetes的协议.

主要方法:

一个新的FISH协议被优化为oomycete检测.
该协议使用无毒的有机化合物.
它绕过了透化步骤的需要,简化了样品的准备.

主要成果:

通过FISH协议,成功地可视化了各种菌体结构.
它对于直接检测和分析殖民植物材料都是有效的.
通过可视化 *Impatiens walleriana* 叶子上的 *Plasmopara destructor* 运动血管体来演示该协议.

结论:

本FISH协议提供了一种快速有效的虫鉴定方法.
简化方法提高了环境和植物病理学研究的可访问性.
直接可视化有助于理解虫生命周期和宿主相互作用.

关键词:

文化独立的可视化鱼类鱼类鱼类光在现场混合化中的光.

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相关概念视频

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...