由Halomonas sp.从挥发性脂肪酸中增强的多3-基酸-co-3-基酸) 生产. YJ01使用2-甲基酸盐循环
Jincan Yang1, Jing Wang2, Xiaoqin Yu1
1School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310018, PR China.
Journal of environmental management
|December 27, 2024
概括
优化挥发性脂肪酸比率,特别是酸盐,酸盐和酸盐,对于最大限度地提高Halomonas sp.中的多3-基酸盐-co-3-基酸盐 (PHBV) 生产至关重要. YJ01.01 年 月 日. 2-甲基酸盐循环途径减轻了酸盐的毒性,增强了PHBV生物合成.
科学领域:
- 微生物生物技术 微生物生物技术
- 聚合物合成的方法
- 代谢工程是代谢工程.
背景情况:
- 挥发性脂肪酸 (VFA) 是聚3-基酸-co-3-基酸 (PHBV) 生产的关键基质.
- 酸是一种VFA,对PHBV生物合成至关重要,但在高度时表现出毒性.
- 优化VFA比率和环境条件对于高效的PHBV合成是必要的.
研究的目的:
- 确定最佳的VFA比率,以最大限度地提高PHBV和3-基瓦莱酸盐 (3HV) 的含量.
- 调查二甲基酸盐循环 (2-MCC) 途径在缓解PHBV合成期间酸盐毒性的作用.
- 阐明控制PHBV生物合成的代谢机制从混合的VFA.
主要方法:
- 种植的哈洛蒙纳斯 sp. 的种植. YJ01具有特定的VFA比率 (乙酸:酸:丁酸 1:4:2).
- 优化碳/ (C/P) 和碳/ (C/N) 的比例.
- 对PHBV生物合成的代谢途径分析.
- 实时光定量聚合酶链反应 (RT-qPCR) 来评估与2-MCC通路和PHBV合成相关的基因表达.
主要成果:
- 在1:4:2的VFA比率下,最大PHBV含量为46.77 wt%和3HV含量为19.24 mol%得到实现.
- 最佳的C/P和C/N比率分别为800-1000和70-90.
- 证明2-MCC途径可以将propionyl-CoA转化为pyruvate,从而降低propionate的毒性并支持PHBV合成.
- 抑制2-MCC通路导致phaC,prpB和prpC基因的显著下调,导致PHBV含量降低.
结论:
- 特定的VFA比率 (1:4:2) 和受控的C/N和C/P比率对于高产量的PHBV生产至关重要.
- 2-MCC途径在酸的排毒中发挥着至关重要的作用,从而增强了Halomonas sp.中的PHBV生物合成. YJ01.01 年 月 日.
- 了解和操纵2-MCC等代谢途径对于克服基质毒性和改善生物聚合物的微生物生产至关重要.
关键词:
碳与的比率是碳与的比率.propionate 的排毒方法挥发性脂肪酸是一种挥发性脂肪酸.聚3-xybutyrate-co-3-xyvalerate) 是一种聚3-xybutyrate-co-3-xyvalerate) 的一种化合物.更多相关视频
相关概念视频
00:46Bioremediation
18.2K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.2K
01:20Fates of Pyruvate
8.3K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.3K
00:53Products of the Citric Acid Cycle
98.2K
The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.
98.2K
01:25Lipid Catabolism
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
01:37The Citric Acid Cycle: Overview
16.1K
In aerobic organisms, the citric acid cycle is the second stage of cellular respiration wherein molecules derived from the breakdown of carbohydrates, proteins, and fats are oxidized into carbon dioxide and energy. This process is also known as the tricarboxylic acid (TCA) cycle as the first product of the cycle, citric acid, contains three carboxyl groups in its structure. Alternatively, this cycle is also referred to as the Krebs cycle, in honor of its discoverer Sir Hans Krebs.
The citric...
The citric...
16.1K
01:28The Citric Acid Cycle: Output
7.6K
The citric acid cycle is termed an amphibolic pathway as it operates both anabolically and catabolically. The cyclic reactions balance the flux of the substrates to provide an optimal concentration of NADH and ATP to the cell.
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...
7.6K