减少脱三乙烯的度接近和通过一个含有Desulfitobacterium的社区减少脱
Lifeng Cao1, Runlei Ge2, Chongwen Shi2
1ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou 311215, China.
Journal of hazardous materials
|December 27, 2024
概括
一种新的Desulfitobacterium菌株耐受高三乙烯 (TCE) 度,这对于污染地下水的有效生物修复至关重要. 这一发现支持使用这些细菌来增强TCE密度非水相液体 (DNAPL) 溶解.
科学领域:
- 环境微生物学环境微生物学
- 生物修复是一种生物修复.
- 污染物水文地质学
背景情况:
- 密度非水相液体 (DNAPL) 污染给地下水清洁带来了挑战.
- 高三乙烯 (TCE) 度接近和水平往往对生物修复微生物有毒.
研究的目的:
- 调查TCE在接近和度的微生物耐受性和脱.
- 在DNAPL源区中确定有效生物修复的微生物候选者.
主要方法:
- 使用16S rRNA基因测序,分离和识别了一种新型的Desulfitobacterium菌株 (THU1).
- 在不同的TCE度下进行微生物群体分析.
- 对THU1菌株的基因组分析,重点是减少性脱酶 (RdhA) 和应激反应基因.
主要成果:
- 一种Desulfitobacterium培养物,包括THU1菌株,在高达8.0毫米的度下将TCE脱化为cis-dichloroethene (cis-DCE).
- 超过4.6毫米的TCE度影响了微生物群落的组成,但没有影响多样性 (香农指数).
- 菌株THU1具有应激反应和调节通路的基因,使其具有高TCE耐受性.
结论:
- 脱硫杆菌表现出代谢灵活性和耐受高TCE度的耐受性.
- 菌株THU1是TCE DNAPL.来源区生物修复的一个有希望的候选人.
- 通过使用耐受性微生物群落增加DNAPL-水度梯度,可以实现增强的TCE溶解.
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