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菌体-等离子体混合体作为环境大肠杆菌中抗生素耐药性的载体

H Ku¹, D Kelk¹, D C Bauer²

¹CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia.

The Science of the total environment

|December 27, 2024

概括

菌体 (菌体) 在大肠杆菌中携带抗微生物耐药性基因 (ARG) 和毒性因子基因 (VFG). 温和菌体是这些基因的重要储存库,菌体等离子体可能会传播β-乳酸盐耐药性.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

抗微生物耐药性基因 (ARG) 和毒性因子基因 (VFG) 对公共健康构成重大威胁.
菌体 (菌体) 是感染细菌的病毒,并可能调解遗传物质的转移.

研究的目的:

研究菌体在大肠杆菌 (E. coli) 中ARG和VFG传播中的作用.
分析不同菌体类型中ARG和VFG的载体模式.
探索由菌体介导的水平基因转移的新机制.

主要方法:

来自国家生物技术信息中心 (NCBI) 数据库的18410个菌素序列的in silico分析.
对环境中的大肠杆菌分离物 (n=60) 进行分析,以检测其中存在益生菌和相关基因.
菌体等离子体 (P-Ps) 和它们的遗传内容的识别和表征.

主要成果:

温和和慢性菌体显示,相比菌体,VFGs的流行率显著更高.
发现温和菌体携带ARG,特别是那些赋予多药和氨基糖化物耐药性的菌体.
环境大肠杆菌隔离了含有ARG和VFG的隐藏的prophages. 鉴定出携带blaCTX-M-15基因的菌体,从而产生β-乳糖抗性.

结论:

菌体作为储存器和潜在载体,在细菌中传播ARG和VFG.
体等离子体是横向基因转移的新机制,可能有助于抗生素耐药性的传播.
需要进一步的研究,以充分阐明菌体介导的基因转移机制.

关键词:

抗微生物耐药性基因是抗微生物耐药性的基因.生物信息学是一种生物信息学.菌体菌体是一种菌体.菌体基因组学菌体基因组学体等离子体病毒性因子基因病毒性因子基因.

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