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北方蛇头 (Channa argus) 的染色体级基因组组装使用PacBio和Hi-C技术

Donglei Sun¹, Haishen Wen¹, Xin Qi¹

¹Key Laboratory of Mariculture (Ocean University of China), Ministry of Education (KLMME), Fisheries College, Ocean University of China, Qingdao, 266003, China.

Scientific data

|December 27, 2024

概括

研究人员对北方蛇头基因组进行了测序,以研究呼吸器官的进化. 这种高质量的基因组组装有助于理解水上空气双模呼吸系统.

科学领域:

进化生物学是进化的生物学.
基因组学就是基因组学.
比较解剖学的比较解剖学.

背景情况:

研究特殊器官的进化是具有挑战性的,因为它们的起源很古老.
北方蛇头 (Channa argus) 提供了一个研究上臂器官的模型,这对于呼吸空气至关重要.
了解像双模呼吸这样的适应的遗传基础是关键.

研究的目的:

为了生成 Channa argus. 的高质量的染色体水平参考基因组.
提供基因资源,用于研究上支臂器官和双模呼吸的演变.
为了研究呼吸空气的鱼类的适应策略.

主要方法:

用于长读数据采集的PacBio HiFi测序.
染色体支架和组装的Hi-C技术.
用于基因预测和质量评估的生物信息分析.

主要成果:

一个712.14 Mb的染色体层次的基因组组合,用于C. argus.
组件固定在24个伪染色体上,具有28.08 Mb的N50支架.
预测了21,643个编码蛋白质的基因,并且具有很高的完整性 (98.6%BUSCOs).

结论:

产生的基因组在连续性和完整性方面比以前的组件有显著的改进.
该资源促进了对水空双模呼吸系统的进化历史和适应机制的研究.
能够更深入地了解鱼类特殊器官进化的遗传基础.

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