病毒外套蛋白降低了同源和异源病毒抑制剂的基因沉默活性
Dóra Pinczés1,2, Réka Sáray1, Katalin Nemes3
1Department of Plant Pathology, Plant Protection Institute, Centre for Agricultural Research, HUN-REN, Budapest, Hungary.
Scientific reports
|December 27, 2024
概括
病毒外蛋白 (CPs) 普遍微调RNA沉默抑制 (RSS) 活动的RNA沉默病毒抑制剂 (VSRs). 这种干扰在不同的病毒家族中观察到,花生特技病毒CP显示最强效应.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 制止RNA沉默 制止RNA沉默
背景情况:
- RNA沉默是一种对病毒的防御机制.
- 病毒RNA沉默抑制剂 (VSRs) 抵消了这种防御.
- 黄瓜马赛克病毒 (CMV) 涂层蛋白 (CP) 之前已被证明可以降低VSR活性.
研究的目的:
- 调查病毒CP在调节VSR活动中的普遍作用.
- 为了比较不同病毒家族的VSRs的RNA沉默抑制 (RSS) 活性.
- 评估来自Bromoviridae和Potyviridae家族的CP和VSR之间的干扰.
主要方法:
- 在Nicotiana benthamiana中经由农细菌介导的短暂表达.
- 对VSR (CMV 2b,PSV 2b,PPV HC-Pro) 和它们的同类/异类CP进行比较分析.
- 在PTGS抑制剂缺乏的CMV突变病毒感染植物中的评估.
主要成果:
- 在所有测试组合中,CP和VSR的联合存在始终降低了RSS活动.
- 这种干扰效应独立于特定的CP-VSR来源,表明它具有普遍作用.
- 花生特技病毒 (PSV) CP 显示了所有三种VSRsRSS活动中最显著的减少.
结论:
- 病毒性CP在微调VSR介导的RNA沉默抑制中发挥着普遍作用.
- CP-VSR相互作用代表了植物病毒进化中的保存机制.
- PSV CP是VSR活动的强大调节器,为病毒与宿主相互作用提供了潜在的见解.
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