在Aspergillus oryzae mycelia中最大化多巴氧化酶活性:对生产优化和潜在的生物医学应用的见解
Sikander Ali1, Hijab Zahra2, M Usman Ahmad2
1Department of Microbiology, Dr. Ikram-ul-Haq Institute of Industrial Biotechnology (IIIB), GC University, 54000, Lahore, Pakistan. dr.sikanderali@gcu.edu.pk.
概括
研究人员使用Aspergillus oryzae菌株优化了多巴氧化酶生产. 添加特定的营养素和稳定剂显著提高了酶活性,为L-多巴基生产铺平了道路.
科学领域:
- 生物技术是生物技术.
- 酶的生产 酶的生产
- 微生物学 微生物学
背景情况:
- 菌 (Aspergillus oryzae) 是一种以产生各种酶而闻名的真菌.
- 多巴氧化酶酶在生物化学途径中起着至关重要的作用.
- 优化酶生产对于工业和临床应用至关重要.
研究的目的:
- 为了生产和优化dopa-oxidase酶使用Aspergillus oryzae.e.的预先生长的质.
- 确定最佳的物理和化学参数,以最大限度地提高多巴氧化酶活性.
- 探索多巴氧化酶在生产L-多巴金方面的潜力.
主要方法:
- 从土壤样本中分离和选Aspergillus oryzae菌株.
- 优化物理参数:pH,温度和注射剂大小.
- 对营养需求的评估:,L-氨酸,L-酸,稳定剂.
- 优化酶和产品产量的化时间.
主要成果:
- 两种A. oryzae分离物 (19和27) 呈现出高的多巴氧化酶活性.
- 最佳条件包括pH值为6,温度为30°C,注射剂大小为1.5%,生物质为2 mg/ml,L-tyrosine为3.75 mg/ml,L-ascorbic acid为8.75 mg/ml.
- 添加像罗歇尔盐,甘油,正酸和乙醇等稳定剂进一步增强了活性.
- 在48小时化后,分别为19号和27号分离物的最大多巴氧化酶活性达到34.18 U/ml和29.02 U/ml.
结论:
- 这项研究成功地优化了A. oryzae.的多巴氧化酶产生.
- 各种添加剂显著增强了多巴氧化酶活性和L-多巴基生产.
- 优化的多巴氧化酶在L-多巴基合成中具有临床应用的潜力.
更多相关视频
10:50Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
Published on: April 1, 2016
10.8K
09:21Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
Published on: February 15, 2019
10.4K
相关概念视频
01:22Bioreactor Controls-III
70
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
70
01:28Methods of Medium Optimization
74
Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...
74
01:25Production of Organic Acids
111
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
111
01:30Production of Pharmaceuticals
99
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
99