通过brassinosteroid信号,OsMAPK6-OsWRKY72模块可以积极调节大米叶的角度
Fuxiang Wang1, Ling Zhang1, Lili Cui2
1Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Fuzhou Branch, National Center of Rice Improvement of China/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou 350003, China; College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
一个名为OsWRKY72的米基因通过影响蛋白质 (BR) 信号传递和膜关节生长来控制叶角. 这种转录因子与OsMAPK6相互作用,影响BR受体OsBRI1的表达,以调节植物结构.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 农业学是一种农业学.
背景情况:
- 叶角是大米植物结构的关键决定因素,影响产量和光合作用效率.
- 铜类固醇 (BRs) 和MAPK信号通路与叶片角度调节有关,但分子机制尚不清楚.
研究的目的:
- 阐明OsWRKY72基因在调节大米叶角方面的作用.
- 研究OsWRKY72在BR信号和植物架构中的功能背后的分子机制.
主要方法:
- 对OsWRKY72突变体和过度表达线的表型分析.
- 层状关节发育的组织学检查.
- 对OsWRKY72与OsBRI1促进体结合的分析.
- 通过OsMAPK6.6对OsWRKY72相互作用和酸化的研究.
主要成果:
- OsWRKY72积极调节大米叶的角度,影响板状关节的发育.
- OsWRKY72突变显示叶角减少和BR不敏感;过度表达线显示扩大角度和BR过敏.
- OsWRKY72直接结合并激活OsBRI1促进体,增强BR信号传输.
- OsWRKY72与OsMAPK6相互作用,并被OsMAPK6酸化,从而提高其活性.
结论:
- OsWRKY72是一种关键的转录因子,通过BR信号传递和板状关节生长调节大米叶角.
- OsMAPK6,OsWRKY72和OsBRI1在协调的路径中起作用,以控制叶片的角度.
- 这项研究提供了对控制米植物架构的分子网络的见解.
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