相关实验视频

Updated: May 8, 2025

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

Published on: February 24, 2021

植物表观遗传学研究中的cytidine类似物,以及其他领域

Eva Dvořák Tomaštíková¹, Ales Pecinka¹

¹Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany, Czech Acad Sci, Šlechtitelů 31, Olomouc 77900, Czech Republic.

Journal of experimental botany

|December 28, 2024

概括

诸如泽布拉林和5-阿扎丁之类的cytidine类似物通过抑制DNA甲基转移酶1 (MET1) 来改变植物表观体. 这些化学抑制剂揭示了植物基因组稳定性和发展的新见解.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
植物生物学植物生物学
分子生物学分子生物学

背景情况:

细胞因子DNA甲基化对于基因调节,基因组稳定性和植物发育至关重要.
了解DNA甲基化需要工具来操纵其水平,包括化学抑制剂.
丁类同类是用于研究植物DNA甲基化的化学工具.

研究的目的:

总结丁类同类化学抑制剂对植物的影响.
阐明这些类似物影响植物表观基因组和发育的机制.
要突出对观察到的表型的DNA破坏性影响和影响.

主要方法:

在植物中使用cytidine类似物 (zebularine,5-azacytidine) 的研究的综述.
分析这些类似物如何被纳入DNA并影响DNA甲基转移酶1 (MET1) 活性.
检查观察到的表观遗传变化,发育变化和DNA-蛋白质交联.

主要成果:

丁类同类物抑制复制性DNA甲基转移酶1 (MET1).
治疗导致植物表观基因组和发育程序的广泛变化.
在DNA和MET1之间形成稳定的DNA-蛋白交叉链接,有助于观察到的表型.

结论:

丁类同类物是植物基因组研究的宝贵工具.
这些化合物在植物生物技术和育种中提供了潜在的应用.
了解它们对DNA的破坏性影响为植物反应提供了新的见解.

关键词:

5 - 甲基细胞氨酸造成的DNA损伤是DNA损伤.通过DNA甲基化.通过DNA-蛋白质交叉链接.丁类型的类似物.表观遗传学是指表观遗传学.植物植物植物植物植物植物.一个重复性的DNA DNA.转录基因沉默是转录的基因沉默.转位子转位子

更多相关视频

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

相关概念视频

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...