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基于结构导向的阿普坦体的双模式侧流测定方法的开发,用于检测沟沟油中的素

Mingwei Qin¹, Ning Ding¹, Pengfei Ma²

¹State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

Biosensors & bioelectronics

|December 28, 2024

概括

研究人员开发了一种高度灵敏的以阿普坦基为基础的侧向流量试验 (Apt-LFA),用于检测食品中的素 (CAP). 这种新的方法利用工程制造的aptamers和Vap@AuNFs来增强色度和光热信号,改善食品安全分析.

科学领域:

分析化学分析化学
生物技术是生物技术.
食品科学食品科学食品科学

背景情况:

基于Aptamer的横向流量测定 (Apt-LFA) 显示出对食品分析的希望.
开发对食品污染物的高度敏感检测方法对于安全至关重要.

研究的目的:

为素 (CAP) 设计一个高亲和度的受体.
开发一个与Vap@AuNFs集成的敏感的Apt-LFA,用于双模式CAP检测.

主要方法:

设计了一种经过修改的阿普坦酶 (Cap-1-2M4),对CAP有增强的亲和力.
在Apt-LFA中利用Vap@AuNFs用于高级色度和光热信号.
实现了用于信号放大的催化发针组件 (CHA) 和外核酶III (Exo III).

主要成果:

获得了高亲和力CAP (0.6197±0.0689nM) 与工程aptamer.
开发了具有双模式 (色度/光热) 检测能力的Vap@AuNFs-Apt-LFA.
获得的低检测极限 (LOD) 为0.043 ng/mL (色度计) 和0.011 ng/mL (光热).

结论:

设计的aptamer和Vap@AuNFs-Apt-LFA为CAP检测提供了一个敏感而高效的平台.
该战略为食品安全分析和便携式检测方法提供了新的方向.

关键词:

基于Aptamer的侧向流量测定.卡普赛辛是一种素.催化式发针组件组合催化式发针组合食品安全食品安全结构指导的阿普坦体.

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