基于融合和液态色谱学结合质谱学的多元组件表征和丹宁片的归因的补充和综合战略
Yu Wang1, Zhengcai Ju2, Linnan Li1
1Shanghai Key Laboratory of Compound Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.
这项研究全面分析了丹宁片 (DNT),一种传统的中医药,使用补充色谱技术. 它确定了11个潜在的定性指标,以改善DNT质量评估.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
- 传统中国医药 传统中国医药
背景情况:
- 丹宁片 (DNT) 是一种用于肝脏和胆囊疾病的传统中医药 (TCM).
- 分析TCM的复杂成分是具有挑战性的,因为不同的化合物极性.
研究的目的:
- 建立一个对DNT多元组件表征的互补策略.
- 确定DNT质量控制的潜在定性指标.
主要方法:
- 使用超高性能融合染色学 (UPCC) 和超高性能液态染色学 (UHPLC).
- 结合色谱与四极飞行时间质谱法 (QTOF-MS) 进行化合物分析.
- 采用网络药理学来验证潜在的指标和相关途径.
主要成果:
- 在DNT中描述了总共247种化合物.
- UPCC-QTOF-MS发现了73种化合物,而UHPLC-QTOF-MS发现了75种化合物.
- 定义了11种化合物作为DNT的潜在定性指标.
结论:
- 开发了一个全面的分析策略,涵盖了DNT的全部极性范围.
- 识别的定性指标可以提高DNT质量评估的准确性.
- 网络药理学证实了指标与DNT治疗效果的相关性.
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