在肺部颗粒状病变中真菌菌的发生率
Sibele Inácio Meireles1, Mariana Vargas Cruz1, Gustavo Palmer Irffi1
1Hospital Sírio Libanês, São Paulo, SP, Brazil.
Clinics (Sao Paulo, Brazil)
|December 28, 2024
概括
包括Mycobacterium tuberculosis (MTB) 和Non-Tuberculosis Mycobacteria (NTM) 在内的真菌细菌在肺部颗粒状病变中被发现. 结合PCR和培养等诊断方法,可以提高及时治疗的检测率.
科学领域:
- 医学微生物学 医学微生物学
- 肺部病理学 肺部病理学
- 传染性疾病 传染性疾病
背景情况:
- 菌根菌感染带来了诊断挑战,特别是在肺小粒状病变中.
- 缺乏用于培养和分子测试的标准化协议,导致诊断延迟.
- 准确识别Mycobacterium结核复合体 (MTB) 和非结核菌 (NTM) 对于有效治疗至关重要.
研究的目的:
- 调查真菌菌种在肺部颗粒状病变中的发生率.
- 评估聚合酶连锁反应 (PCR) 对形式氨基固化嵌组织 (FFPE),直接培养和齐尔-尼尔森 (ZNS) 染色的诊断贡献.
- 评估结合多种诊断方法用于检测真菌菌菌的实用性.
主要方法:
- 对336例肺颗粒状病变病例的回顾性分析.
- 使用Ziehl-Neelsen (ZNS) 染色检测真菌菌菌.
- 从临床标本中直接培养菌根菌.
- 在FFPE组织中检测MTBDNA的聚合酶链反应 (PCR).
主要成果:
- 在16.72% (ZNS) 到20.20% (培养) 的病例中检测到真菌菌.
- 在阳性培养的65.00%中发现了MTB,在32.50%中发现了NTM.
- 在17.54%的FFPE样本中,PCR检测到MTB DNA.
- 确定了各种NTM物种,包括M. avium和M. kansasii.
结论:
- 菌根菌是肺部粒状病变中的重要病原体.
- 结合培养,PCR和组织学的综合诊断方法提高了菌根菌检测.
- 通过补充方法及时准确诊断对于指导患者治疗至关重要.
更多相关视频
10:04Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates
Published on: September 5, 2017
18.8K
09:58Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry LCM-LC/MS
Published on: April 18, 2018
9.0K
相关概念视频
Pulmonary Tuberculosis II
211
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
211
Pulmonary Tuberculosis III
303
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
The first classification is based on the development of the disease, and it includes the following categories:
303
Pulmonary Tuberculosis IV
133
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
133
Pulmonary Tuberculosis I
220
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
220
Pulmonary Tuberculosis V
170
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
170
