相关实验视频

Updated: Jun 4, 2025

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

在温度应激下,对Castanea mollissima中ABC基因家族的全基因组识别,分谱,进化扩张和表达分析

Liyang Yu¹, Yujuan Tian², Xiangyu Wang³

¹Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, Hebei, China; Hebei Key Laboratory of Horicultural Germplasm Excavation and Innovative Utilization, College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Changli, 066600, Hebei, China.

Plant physiology and biochemistry : PPB

|December 28, 2024

概括

研究人员在栗子基因组中确定了164个ATP结合盒 (ABC) 基因,揭示了像CmABCG29c和CmABCB11e这样的关键基因,这些基因参与了温度应激反应和植物适应.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
环境压力生理学环境压力生理学

背景情况:

ATP结合盒 (ABC) 载体对于植物的恒温和适应至关重要.
在栗子 (C. mollissima) 中理解ABC基因家族是有限的.
这项研究重点关注C. mollissima ABC基因的特征及其在温度压力中的作用.

研究的目的:

确定和描述C. mollissima.中的ABC基因家族.
研究CmABC基因在温度压力反应中的作用.
为了确定参与温度应激适应的候选基因.

主要方法:

在C. mollissima.中对164个ABC基因进行全基因组识别和分类.
对线分析以确定基因家族扩张机制.
在温度压力下对形态和生理变化的分析.
转录组分析 (RNA-seq) 和权重基因联合表达网络分析 (WGCNA).
使用RT-qPCR验证基因表达.

主要成果:

164个CmABC基因被确定并分为八个子家族.
分散重复是CmABC基因家族扩张的主要驱动因素.
温度压力显著影响了光合作用指标和SOD酶活性.
在压力下观察到CmABC基因的明显表达模式.
确定CmABCG29c和CmABCB11e是温度应激反应的关键候选物.

结论:

该研究提供了CmABC基因的全面目录,并对其演变的洞察力.
在C. mollissima对温度压力的反应中,CmABC基因起着重要的作用.
CmABCG29c和CmABCB11e是改善C. mollissima.温度应激耐受性的潜在目标.

关键词:

这是ABC基因家族的基因家族.抗氧化剂酶是一种抗氧化酶.卡斯塔尼亚海 (Castanea mollissima) 是一个很小的鱼类.在RNA-seqqq.温度压力是因为温度压力.

更多相关视频

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

Measurements of Physiological Stress Responses in C. Elegans

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

相关概念视频

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.