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在氧化还原活性纳米复合物中对NADH和脱酶酶的安培度测定

Zachary Lucio-Rivera¹, Gisela Sanchez¹, Waldemar Gorski¹

¹Department of Chemistry, University of Texas at San Antonio, TX, 78249, USA.

|December 28, 2024

概括

一种新型电极材料CNTNH2-GDI-Azure (CGA) 显著改善了人类血清中NADH检测和酶活性测量. 这种稳定的CGA电极为诊断人类疾病提供了更高的灵敏度和更少的干扰.

科学领域:

电化学电化学电化学
纳米材料科学科学纳米材料科学
生物医学诊断生物医学诊断

背景情况:

尼古丁胺胺氨基二核酸 (NADH) 是各种代谢过程和疾病的关键生物标志物.
准确和敏感地检测NADH和NADH依赖酶对于临床诊断至关重要.
传统的电极材料经常受到高超电位和矩阵干扰的影响,限制了它们的诊断效用.

研究的目的:

开发一种新的,高度敏感和稳定的电极材料,用于电化学测定NADH.
研究这种新电极材料在生物样本中量化NADH依赖酶的应用.
在灵敏度,稳定性和减少干扰方面评估新电极的性能.

主要方法:

氨基化碳纳米管 (CNTNH2) 与谷物二甲基 (GDI) 和Azure染料的共价修饰,以创建CNTNH2-GDI-Azure (CGA) 纳米复合材料.
用于电极制造的CGA与奇托纳米复合物的配方.
在人血清样本中使用CGA电极对NADH和NADH依赖酶 (LDH,MDH) 的电化学分析.
与传统电极相比,对电极稳定性和干扰减少的评估.

主要成果:

该CGA电极显著降低了超过0.40V的NADH氧化过量的潜力,最大限度地减少了矩阵干扰.
对NADH,LDH和MDH的检测极限分别低至0.6μM,1.5U L-1和0.5U L-1.
稳定的NADH电流 (∼103%) 维持了10个小时,与传统电极的衰变电流形成鲜明对比.
用快速,内部校准的测定方法,在临床相关范围内准确量化了酶活性.

结论:

开发的CGA电极材料对NADH和NADH依赖酶的确定表现出了卓越的性能.
它的低超电位,高灵敏度和出色的稳定性使其适合于现实生活样本分析.
通过生物标志物检测,CGA为开发先进的生物传感器提供了一个有前途的平台,用于通过生物标志物检测诊断人类疾病.

关键词:

电压计传感器和生物传感器确定NADH的方法脱酶酶的电化学测定降解氧调解的调解方法合成电极材料的合成

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