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早期内分体的酸性pH值控制了SARS-CoV-2在宿主细胞中的运输
Perla Fares1, Mariam Duhaini1, Suvranta K Tripathy1
1Department of Natural Sciences, University of Michigan-Dearborn, Dearborn, Michigan, USA.
The Journal of biological chemistry
|December 28, 2024
概括
在早期内分体 (EEs) 中增加-质子交换器9 (NHE9) 表达会提高它们的pH值,阻碍SARS-CoV-2的传输,并降低病毒感染力. 这表明NHE9是阻止病毒进入的治疗点.
科学领域:
- 细胞生物学 细胞生物学
- 病毒学 病毒学
- 分子医学是分子医学.
背景情况:
- 内细胞分裂是SARS-CoV-2进入宿主细胞的关键途径.
- 早期内分泌体 (EEs) 成熟为晚期内分泌体 (LEs),这个过程取决于pH值变化和运动蛋白质运输.
- 和质子交换器9 (NHE9) 与严重的COVID-19有关,并调节内体PH.
研究的目的:
- 为了研究早期内体光pH在SARS-CoV-2传输中的作用.
- 确定NHE9如何影响早期内体运输和SARS-CoV-2感染性.
- 为了确定阻止病毒进入的潜在治疗点.
主要方法:
- 研究了NHE9表达对早期内体 (EE) 光线pH的影响.
- 使用显微镜研究了改变EE pH对含有SARS-CoV-2的囊泡的运输的影响.
- 分析了氨酸-3-酸盐 (PI3P) 在调解NHE9对EE运输的影响中的作用.
主要成果:
- 增加NHE9表达导致早期内分泌体 (EEs) 的酸性 (性) 程度降低.
- 这种 alkalization 损害了 EEs 沿微管道的运动,减少了它们的速度和运行长度.
- 在高NHE9细胞中EEs上的持久酸-3-酸盐 (PI3P) 与改变的运动蛋白征集有关,并阻碍了周核运输.
- 减少EE传输到晚期内体导致SARS-CoV-2感染率降低.
结论:
- 早期内体光pH值是SARS-CoV-2传输和进入的关键调节者.
- 通过NHE9介导的内体化破坏了依赖于EE运动蛋白的输送,损害了病毒传递.
- NHE9代表了一种有前途的治疗标,可以抑制SARS-CoV-2宿主细胞的进入.
关键词:
在 COVID-19 疫情中,这就是NHE99.这就是SARS-CoV-2病毒.在SLC9A9A9A9A9A9A9A9A9A内细胞贩运,内细胞贩运.细胞内分泌症 (endocytosis) 是一种细胞内分泌症.它们的pH值为pH.- 质子交换是一种交换.更多相关视频
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