在诱导生理机械拉伸下,人类大动脉光滑肌细胞中的基因表达调节
Amira Ben Hassine1, Claudie Petit1, Mireille Thomas1
1Mines Saint-Etienne, Université Jean Monnet, INSERM, U 1059 SAINBIOSE, Saint-Etienne, 42023, France.
Scientific reports
|December 29, 2024
概括
动态拉伸迅速调节人类大动脉光滑肌细胞 (AoSMCs) 的细胞外基因基因. 这种机械刺激对AoSMC机械生物功能和心血管健康至关重要.
科学领域:
- 心血管生物学 心血管生物学
- 细胞机械生物学 细胞机械生物学
- 分子生物学分子生物学
背景情况:
- 大动脉光滑肌细胞 (AoSMCs) 对于血管完整性至关重要.
- 机械力,如动态拉伸,显著影响AoSMC的行为.
- 了解AoSMC机械传导是心血管健康的关键.
研究的目的:
- 为了研究AoSMCs对生理动态拉伸的体外基因表达反应.
- 确定在AoSMCs中由机械应力调节的特定基因.
- 阐明脉动拉伸在AoSMC机械生物功能中的作用.
主要方法:
- 人类初级AoSMC在4到72小时内以1Hz的频率进行9%的动态拉伸.
- 用定量实时PCR (RT-qPCR) 分析基因表达.
- 未拉伸的AoSMC在相同的培养条件下作为对照.
主要成果:
- 动态拉伸在4小时内快速上调关键基因 (COL1A1,FBN1,LAMA5,TGFBR1,TGFBR2).
- 上调的基因与细胞外矩阵 (ECM) 形成和引力调节有关.
- 拉伸的AoSMC保持稳定的基因表达,而对照细胞显示变异.
结论:
- 脉冲拉伸在调节AoSMCs中的基因表达方面发挥着关键作用.
- 动态机械线索对于维持AoSMC机械生物学的平衡是必不可少的.
- 研究结果提供了关于心血管健康和疾病机制的见解.
更多相关视频
08:28Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
Published on: February 15, 2022
3.5K
11:47Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall
Published on: July 6, 2022
3.0K
相关概念视频
Cell-matrix's Response to Mechanical Forces
2.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
2.4K
Smooth Muscle Contraction
2.0K
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
2.0K
