VPS28通过在牛乳腺上皮细胞中通过ubiquitination调节甘油三合成
Lily Liu1,2, Jinhai Wang3, Xianrui Zheng4
1College of Biological and Food Engineering, Southwest Forestry University, Kunming, 650224, China. v1lliu34@ed.ac.uk.
Scientific reports
|December 29, 2024
概括
VPS28蛋白调节牛奶脂肪合成,通过影响全方位化和影响CD36和ADFP等关键脂质代谢基因的表达. 调节VPS28对牛乳细胞和小鼠模型中的甘油三生产产生影响.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- VPS28是ESCRT复合体的一个子单元,涉及到泛化,这是蛋白质降解的关键过程.
- 基化在调节脂质新陈代谢方面发挥着作用.
- VPS28在牛奶脂肪合成中的确切作用尚不清楚.
研究的目的:
- 研究VPS28对参与牛奶脂肪合成的基因表达的影响.
- 确定VPS28在牛乳腺上皮细胞 (MAC-T) 中的甘油三 (TG) 合成中的作用.
主要方法:
- 在MAC-T细胞中调节VPS28表达 (敲击和过度表达) 使用lentivirus介导的技术.
- 测量甘油三合成,脂肪酸载体 (CD36),脂肪分化相关蛋白 (ADFP),无素 (UB) 水平和蛋白质酶活性.
- 抑制无素-蛋白酶体系统和内体-溶酶体通路.
- 在小鼠模型中进行体内验证.
主要成果:
- VPS28 knockdown 上调了 CD36 和 ADFP,增加了 TG 和脂肪酸的产生,并增加了 UB 水平和减少了蛋白质酶活性.
- VPS28的过度表达增加了CD36,但对ADFP和TG有轻微的影响,趋势是减少脂质滴和增加UB表达和蛋白质酶活性.
- 抑制降解途径增强了CD36,ADFP和TG水平,改善了细胞活力. 在体内,VPS28 Knockdown增加了乳腺CD36,ADFP,UB,TG含量和脂质滴,没有任何不良影响.
结论:
- VPS28在调节通过ubiquitination路径的甘油三合成中发挥着关键作用.
- VPS28通过调节CD36和ADFP的表达来影响牛奶脂肪的产生.
- 这些发现为牛奶脂肪合成的分子机制提供了新的见解.
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