动态转录组学揭示了Artemisia annua在寒冷压力下的艺术素和烯胺生物合成途径的并行转录调节
Yunxiao He1, Wenjing Zhang1, Xianghua Zuo1
1National & Local United Engineering Laboratory for Chinese Herbal Medicine Breeding and Cultivation, School of Life Sciences, Jilin University, Changchun, Jilin Province, People's Republic of China.
寒冷压力促进了Artemisia annua.中的素素前体的合成. 然而,长时间的感冒会抑制最终的美素路径,增加相关化合物.
科学领域:
- 植物科学 植物科学
- 代谢工程是代谢工程.
- 生物化学 生物化学
背景情况:
- 寒冷压力是影响植物二次新陈代谢的重要非生物因素.
- 阿尔特米西亚年 (Artemisia annua) 能产生阿尔特米西宁,这是一种有价值的抗疟疾化合物.
- 了解对寒冷压力的代谢反应对于优化植物性生产至关重要.
研究的目的:
- 在寒冷压力下调查Artemisia annua中二次代谢物合成的动态变化.
- 为了阐明在低温条件下阿尔特米西宁和烯胺生物合成的调节机制.
- 为了确定关键的基因和途径,涉及寒冷诱导的代谢变化.
主要方法:
- 在寒冷压力下对Artemisia annua叶子进行动态转录组分析.
- 代谢物量化以测量二次代谢物的积累.
- 对生物合成途径中关键酶和转录因子的基因表达模式的分析.
主要成果:
- 寒冷压力增加了总有机碳的积累,为二次代谢产物提供了前体.
- 短期暴露在寒冷中暂时增加了斯蒙酸,增强了艺术胺素的合成.
- 长时间的冷持续的烯基胺合成 (林宁,黄类,氨酸),但抑制了最终的胺素路径,增加了二胺酸和胺酸.
结论:
- 寒冷压力在Artemisia annua.中差异调节了素和烯胺生物合成途径.
- 这些通路的协调转录调节在低温条件下发生.
- 研究结果提供了通过管理寒冷应激反应来优化素生产的见解.
更多相关视频
11:16A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
Published on: July 22, 2017
11:27A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
Published on: August 25, 2018
相关概念视频
Responses to Heat and Cold Stress
Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Biological Clocks and Seasonal Responses
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Adaptations that Reduce Water Loss
