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Updated: Jun 4, 2025

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
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Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

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不稳定基因的稳定组合优于RT-qPCR数据规范化的标准参考基因

Anis Djari1, Guillaume Madignier1,2, Christian Chervin1

  • 1Laboratoire de Recherche en Sciences Végétales, Equipe Génomique et Biotechnologie des Fruits, UMR 5546, CNRS, UPS, Toulouse INP, Université de Toulouse, Toulouse, France.

Scientific reports
|December 29, 2024
PubMed
概括

找到基因的稳定组合,而不是单个的基因,可以改善定量聚合酶链反应 (qPCR) 数据的规范化. 这种新方法使用RNA测序数据来识别最佳基因组,用于准确的基因表达分析.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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