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在智利圣佩德罗德拉帕斯的废水中追踪SARS-CoV-2变种

Andressa S Reis¹, Christian Castro¹, Paulina Assmann²

¹Centro de Vigilancia de Aguas Residuales, Centinela Biobío, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile.

Journal of water and health

|December 29, 2024

概括

在智利,废水监测检测到SARS-CoV-2变种,包括Lambda和Delta. 这种快速,具有成本效益的方法补充了临床数据,在临床病例出现之前识别了变异.

科学领域:

环境微生物学环境微生物学
病毒学病毒学
公共卫生监督是对公共卫生的监督.

背景情况:

人类便中存在SARS-CoV-2,这使得基于废水的监测 (WBS) 成为可能.
新兴变种需要持续监测,以控制传染病的传播.
WBS提供了一种补充疾病检测临床监测的方法.

研究的目的:

在废水中检测SARS-CoV-2及其变体.
在智利圣佩德罗德拉帕斯评估WBS变种监测.
将WBS发现与临床数据和测序进行比较.

主要方法:

废水样本是在2021年1月至11月收集的.
使用聚乙烯糖醇方法的病毒度,回收率为~11%.
针对SARS-CoV-2和变种 (阿尔法,贝塔,玛,兰巴达,三角形) 的RT-qPCR;与Illumina测序进行比较.

主要成果:

WBS检测到SARS-CoV-2和变体敏感且与临床数据一致.
在感染波中,Lambda和Delta变种最常见.
在确诊临床病例之前,在废水中检测到了一些变体.
照明测序显示废水样本的基因组覆盖不足.

结论:

基于废水的监测是一种快速,具有成本效益的工具,用于检测SARS-CoV-2和突变.
WBS提供了宝贵的公共卫生见解,特别是在高临床负担或资源限制期间.
需要改进的测序方法来进行全面的废水基因组监测.

关键词:

在 COVID-19 疫情中,RT-qPCRR 是一个人.在SARS-CoV-2变种中.公共卫生公共卫生.污水监测污水的监测情况

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