使用UV-LED通过无活化,解体和剥离来有效管理先前存在的生物膜
Liang Shen1, Wenqi Chen1, Jinyu He1
1Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, the Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China.
Journal of hazardous materials
|December 29, 2024
概括
紫外发光二极管 (UV-LED) 辐射通过降解细胞外聚合物物质 (EPS) 有效地使大肠杆菌 (E. coli) 生物膜失活. 将UV-LED处理与液压剪切相结合,可以增强水系统中的生物薄膜去除.
科学领域:
- 环境微生物学环境微生物学
- 水处理技术水处理技术.
- 消毒科学是消毒的科学.
背景情况:
- 生物膜在水处理和配送系统中带来了重大挑战.
- 紫外线发光二极管 (UV-LED) 辐射是一种有前途的无化学品消毒方法.
- 有限的研究存在于UV-LED对已建立的生物膜的有效性.
研究的目的:
- 对预制的大肠杆菌 (E. coli) 生物膜进行UV-LED辐射的性能和机制的研究.
- 探索不同辐射条件对生物膜失活的影响.
- 了解细胞外聚合物质 (EPS) 在UV-LED介导的生物膜破坏中的作用.
主要方法:
- 预制的大肠杆菌生物膜暴露在不同波长 (268,275,312,370 nm) 的UV-LED辐射中.
- 多种辐射参数,包括流量和辐射强度.
- 在辐射后对细胞外聚合物质 (EPS) 结构和化学特性进行分析.
- 在被辐射的生物膜上应用额外的液压剪切.
主要成果:
- 波长为275纳米的波长显示出最佳的无活化,实现了生物膜结合的大肠杆菌减少3.2log.
- 辐射量和辐射时间被确定为影响日志不激活的并联变量,修改了邦森-罗斯科互惠定律.
- 紫外线-LED辐射导致EPS矩阵内的多糖和蛋白质的氧化降解,破坏了生物膜结构.
结论:
- 紫外线-LED照射,特别是在275nm,有效地使大肠杆菌生物膜失活.
- 对EPS的降解是UV-LED生物膜控制的主要机制.
- 紫外线LED处理和液压剪切的协同应用可以提高生物膜去除效率.
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