使用全基因组再测序识别关键基因和与野猪精子结能力相关的变异
Wenjun Wang1, Yifan Bai2, Xiaokang Wang2
1Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
International journal of biological macromolecules
|December 29, 2024
概括
研究人员确定了影响野猪精子承受冷保存的能力的遗传因素. 发现了关键的基因和分子标记物,为改善野猪繁殖实践和精子质量提供了潜力.
科学领域:
- 动物育种和遗传学动物育种和遗传学
- 生殖生物学 生殖生物学
- 低温生物学 低温生物学
背景情况:
- 猪精子的冷保存会导致结构和功能损伤,导致精子质量下降.
- 单个野猪精子在对冷保存的反应中表现出显著的变化.
研究的目的:
- 为了研究猪精子冷性差异的遗传基础.
- 识别与精子冷存活相关的候选基因和分子标记物.
主要方法:
- 进行了全基因组再测序和FST分析,对具有良好的 (GSF) 和差的 (PSF) 精子结能力的野猪进行了测试.
- 使用MassARRAY基因型和一般线性模型来分析遗传变异.
主要成果:
- 在GSF和PSF野猪之间的5632个基因中观察到显著的遗传差异.
- 他们确定了35个候选基因,包括RNF8,PACRG,CADM1,SPAG16,PPP3CA和MFF.
- 在候选基因中检测到9种与精子结能力有显著相关的遗传变异 (七种SNP,两种插入).
结论:
- 遗传因素对野猪精子的冷性起着至关重要的作用.
- 已识别的候选基因和分子标记物有可能用于选择性猪繁殖,以增强精子冷生存.
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