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相关概念视频

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.7K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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The Replisome03:01

The Replisome

32.4K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
32.4K
Homologous Recombination02:31

Homologous Recombination

49.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
49.7K

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相关实验视频

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A Customizable Protocol for String Assembly gRNA Cloning STAgR
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A Customizable Protocol for String Assembly gRNA Cloning STAgR

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惰性片驱动的寡核酸组件组件

Andrew A Mishin1, Tobin Groth1, Richard E Green1,2

  • 1Claret Bioscience LLC, 100 Enterprise Way, Suite A102, Scotts Valley, CA 95066, United States.

Synthetic biology (Oxford, England)
|December 30, 2024
PubMed
概括

分合成是一种新的单管方法,用于使用分驱动的反应组装寡核酸. 这种技术有效地连接较长的DNA片段,使合成生物学和基因合成中的应用成为可能.

科学领域:

  • 分子生物学分子生物学
  • 合成生物学 合成生物学
  • 生物技术是生物技术.

背景情况:

  • 传统的寡核酸组装方法,如聚合酶链组装和酶链组装,需要短,高度净化的DNA片段.
  • 这些方法可能受到广泛净化的需要和处理众多短的寡核酸的复杂性所限制.

研究的目的:

  • 引入一种名为Splynthesis的新型体外方法来进行寡核酸碎片组装.
  • 为了提供一种更有效和精简的方法,用于组装更大的DNA片段,使用一个单管, splint驱动的反应.

主要方法:

  • 分裂合成使用标准脱盐的"紧密"寡核酸 (大约150个核酸) 和更短的"片"寡核酸.
  • 片寡核酸含有5'和3'阻断修饰,以防止不必要的结合和放大.
  • 这种方法采用一个单管,刺驱动的组装反应来连接DNA片段.

主要成果:

  • 通过Splynthesis方法,成功地组装了741-bp基因片段.
  • 组装的DNA片段使用标准的分子生物学技术进行了验证,包括长期阅读的牛津纳米孔测序.
  • 由此产生的产品通过分子克隆和桑格测序被证实是可克隆的.

结论:

关键词:
基因分离器的 DNA 分离器.基因组合组合的基因组合.基因片段 基因片段 基因片段这就是Oligos Oligos.

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  • 分裂合成提供了一种强大而有效的方法来组装寡核酸碎片.
  • 该技术适用于各种领域,包括合成生物学,定向进化和功能蛋白质测试.
  • 支骨合成也可以适应基于支骨的酶连锁反应试验.