相关实验视频

Updated: May 10, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

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一个自催化平台,结合非线性杂交链反应和DNA酶来检测微RNA

Hongbo Zhang1, Xiuen Cao1, Qubo Zhu1

  • 1Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China.

Bio-protocol
|December 30, 2024
PubMed
概括

这项研究引入了一种新的方法来检测miRNA-21,使用分枝杂交链反应 (bHCR) 和DNAzyme技术. 这种方法为早期疾病诊断提供了一个敏感,快速和特定的替代方案.

关键词:
分支杂交连锁反应 (bHCR) 是一种这就是DNAzyme DNA酶.微RNA (miRNA) 是一种微型RNA.实时定量聚合酶连锁反应 (RT-qPCR) 是一种实时定量聚合酶连锁反应.这就是miRNA-21-21的原因.

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相关概念视频

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...