马产腺规则中的前列腺素通路:子宫内膜异位症的进展和进展
Katarzyna K Piotrowska-Tomala1, Anna Z Szóstek-Mioduchowska1, Ewa M Drzewiecka1,2
1Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research Polish Academy of Science, Olsztyn, Poland.
马匹的子宫内膜异位症改变了前列腺素 (PG) 基因表达和子宫肌中的受体活性. 这导致异常的子宫收缩,这表明在这种情况下肌肉功能受到损害.
科学领域:
- 生殖生物学 生殖生物学
- 马类医学 马类医学
- 分子内分泌学分子内分泌学
背景情况:
- 前列腺素 (PG) 是哺乳动物肌体收缩的关键调节剂.
- 子宫内膜异位,一种影响马子宫内膜异位的疾病,可能会影响子宫肌功能,但其机制尚未完全理解.
研究的目的:
- 调查由子宫内膜异位症影响的马体内膜异位症中PG合成酶和受体的mRNA转录的变化.
- 为了确定PGE2和PGF2α对子宫内膜异位症的母马的肌体收缩性的影响.
主要方法:
- 定量PCR (qPCR) 用于分析马匹肌肉学中的前列腺素内氧化合成酶 (PTGS2),PGE2合成酶 (PTGES),PGF2α合成酶 (PTGFS) 和它们的受体 (PTGER1-4) 的mRNA水平.
- 用对PGE2和PGF2α的响应测定异比收缩传感器来评估肌肉的收缩性.
- 采集的样本来自中期白色和卵泡阶段的母马,按子宫内膜组织学分类 (肯尼和多伊格分类I,IIA,IIB,III).
主要成果:
- 子宫内膜异位与肌体中PTGES,PTGER1,PTGER2和PTGFS的改变的mRNA转录相关,因雌性周期阶段和子宫内膜类别而异.
- 在卵泡期内,PGE2降低了患有子宫内膜异位症 (IIA,IIB,III类) 的母马的肌膜收缩力.
- PGF2α具有差异效应,增加IIA类的收缩,减少IIB类子宫内膜的收缩.
结论:
- 子宫内膜异位症的进展与参与PG合成和信号传递的基因,特别是PTGER1和PTGER2.的肌肉表达的显著变化有关.
- 患有子宫内膜异位症的母马对前列腺素表现出异常的肌体反应.
- 这些发现表明,随着子宫内膜异位症的进展,子宫肌功能可能会受到损害.
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