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相关概念视频

Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
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Extraction: Partition and Distribution Coefficients01:14

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The distribution law or Nernst's distribution law is the law that governs the distribution of a solute between two immiscible solvents. This law, also known as the partition law, states that if a solute is added to the mixture of two immiscible solvents at a constant temperature, the solute is distributed between the two solvents in such a way that the ratio of solute concentrations in the solvents remains constant at equilibrium.
For extracting a solute from an aqueous phase into an...
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Overview of VSEPR Theory
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相关实验视频

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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在SELEX中对分区严格性的定量表征

An T H Le1,2, Eden Teclemichael1,2, Svetlana M Krylova1,2

  • 1Department of Chemistry, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada.

JACS Au
|December 30, 2024
PubMed
概括

通过指数式丰富 (SELEX) 控制联体的系统演化中的严格性至关重要. 这项研究引入了粘合剂与非粘合剂比率 (BNR) 来定量测量严格性,防止SELEX故障.

科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 生物化学 生化学

背景情况:

  • 通过SELEX选择aptamer需要严格的条件,高亲和度的结合剂.
  • 在SELEX中过度严格增加了选择失败的风险.
  • 目前的SELEX严格性控制依赖于直觉,因为缺乏定量措施.

研究的目的:

  • 为SELEX严格性制定一个定量衡量标准.
  • 建立一个防止SELEX故障的标准.
  • 为了更合理地控制aptamer选择过程.

主要方法:

  • 使用粘合剂与非粘合剂比率 (BNR) 量化严格性.
  • 开发了一种实验方法,通过定量PCR来确定BNR.
  • 分析了理论方面,并对两个蛋白质标进行了SELEX实验.

主要成果:

  • 引入了BNR作为SELEX严格性的定量衡量标准,其中较低的BNR表明更高的严格性.
  • 建立了SELEX无故障标准:保持BNR明显大于零.
  • 证明了反向BNR可用于量化严格性并指导SELEX进展.

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结论:

  • 该BNR提供了一种合理的方法来控制SELEX的严格性和避免失败.
  • SELEX无故障标准和BNR适用于其他人工进化方法.
  • 定量严格度测量提高了阿帕特默选择的可靠性和成功率.