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从奶牛中分离的Streptococcus agalactiae的致病性和耐药性表征

L J Jiang¹, H R Liu¹, Z Y Liu²

¹Department of Customs Inspection and Quarantine, Shanghai Customs College, Shanghai, China.

Polish journal of veterinary sciences

|December 30, 2024

概括

乳牛中的Streptococcus agalactiae对四环素和青素具有很高的耐药性,但对几个关键抗生素仍然敏感. 病毒性基因在动物模型中对其病原性有显著的贡献.

科学领域:

兽医微生物学兽医微生物学
抗微生物耐药性抗微生物耐药性
动物传播疾病动物传播疾病

背景情况:

甲状腺杆菌 (S. agalactiae) 是一种影响乳牛,牛奶产量和公共健康的动物传染病原体.
与其他乳腺炎病原体相比,对乳牛中的S. agalactiae的研究范围较小.
了解抗生素耐药性和毒性对于管理S. agalactiae感染至关重要.

研究的目的:

为了确定乳牛中S. agalactiae的抗生素耐药性概况.
使用体内模型评估S. agalactiae分离物的致病性.
研究病毒性基因与病原性之间的相关性.

主要方法:

使用微稀释进行抗生素敏感性测试.
聚合酶连锁反应 (PCR) 用于识别毒性基因.
在Galleria mellonella幼虫和小鼠模型中进行体内致病性评估.

主要成果:

在S. agalactiae的分离物中,它们对链胺素,美罗胺,西普罗夫洛素,克林达米辛,素和克洛西林敏感.
对四环素 (70%),青素 (50%) 和多西环素 (30%) 观察到高耐药率.
所有分离物携带cfb基因,其中9个分离物拥有4个额外的毒性基因,与致病性模型中的高死亡率相关.

结论:

乳牛S. agalactiae对四环素和青素等常见抗生素具有显著的耐药性.
病毒性因素,包括cfb基因和其他因素,在S. agalactiae的病原性中发挥着关键作用.
研究结果提供了对环境抗生素耐药性和乳制品环境中的S. agalactiae毒性的见解.

关键词:

链球菌的 agalactiaeae 是一个细菌.在体内活体模型.病原性病原性病原性毒性毒性是一种毒性.

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