重复的精液射精对精子质量和犬精液的选择生物化学标记物的影响
R Strzeżek1, M Lecewicz1, I Orzeł1
1Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland.
Polish journal of veterinary sciences
|December 30, 2024
概括
狗精液质量在禁欲后反复采样24小时后会改善,但每天采集精子数量会减少. 这影响了人工授精的成功.
科学领域:
- 生殖生物学 生殖生物学
- 兽医科学 兽医科学 兽医科学
- 动物繁殖 动物繁殖
背景情况:
- 狗精液质量对于人工授精和保存至关重要.
- 长时间的性禁欲可能会对精液参数产生负面影响.
- 了解反复射精的影响对于优化精液采集协议至关重要.
研究的目的:
- 为了评估狗精液质量和生物化学标记,在5天内重复24小时间隔采样.
- 评估性禁欲持续时间对精液参数的影响.
- 确定用于犬类人工授精的最佳采样策略.
主要方法:
- 在连续5天内收集了6只狗的射精,每隔24小时.
- 评估的射精量,精子总数,运动性 (TMOT,PMOT),精子等离子体膜完整性 (SPMI) 和线粒体膜潜力 (MMP).
- 在精子血中测量ATP含量,总蛋白质含量 (TPC) 和酶活性 (AAT,AP,AcP).
主要成果:
- 排精量和精子数量随着连续的每日采样而下降.
- 移动性,膜完整性,线粒体潜力和ATP含量在采样期间增加.
- 精液等离子体蛋白质和酶水平 (AAT,AP,AcP) 降低.
结论:
- 在5天内反复射精会改变狗精液质量和生物化学特征.
- 戒精后的初始精液质量可能在繁殖目的下是不理想的.
- 虽然每天取样可以改善一些参数,但它减少了总体的精子产量,可能会限制人工授精剂量.
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