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腹羊中的大肠杆菌:流行率,毒性和抗生素耐药性
T Gurjar1, R Singathia1, D K Sharma1
1Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur, Rajasthan University of Veterinary and Animal Sciences (RAJUVAS), Rajasthan, India.
Polish journal of veterinary sciences
|December 30, 2024
概括
这项研究研究了腹羊中的大肠杆菌,在15.22%的羊群中发现了产生石毒素的大肠杆菌 (STEC),在76.09%的羊群中发现了集性大肠杆菌. 大多数隔离品对常见抗生素有抗药性.
科学领域:
- 兽医微生物学 兽医微生物学
- 细菌学 细菌学是一门学科.
- 分子流行病学分子流行病学
背景情况:
- 大肠杆菌 (大肠杆菌) 是羊羔腹的重要原因之一.
- 了解大肠杆菌在牲畜中的毒性因素和抗菌素耐药性概况对于动物健康和公共安全至关重要.
研究的目的:
- 为了确定腹羊羔中不同大肠杆菌病型的流行率.
- 描述孤立大肠杆菌菌株的毒性和抗菌素耐药性基因.
- 评估大肠杆菌分离物的抗微生物敏感性模式.
主要方法:
- 从腹的羊羔收集便样本.
- 对大肠杆菌的隔离和鉴定.
- 聚合酶链反应 (PCR) 用于检测毒性基因 (stx1,stx2,eae,lt,st) 和抗菌素耐药性基因 (tetA,blaTEM).
- 对15种抗生素进行抗微生物敏感性测试.
主要成果:
- 从61个样本中检测出46个大肠杆菌分离体.
- 在15.22% (n=7) 检测到产生毒素的大肠杆菌 (STEC).
- 在8.70% (n=4) 发现了非典型的肠病原性大肠杆菌 (EPEC).
- 输入出血性大肠杆菌 (EHEC) 在2.17% (n=1) 确诊.
- 在76.09% (n=35) 个案中发现了肠杆菌 (EAEC) 肠内聚合型病变型.
- 所有的分离物都是非中毒性大肠杆菌 (ETEC).
- 观察到对青素-G,头素和亚齐菌素的高耐药性.
- 对氨基醇,牛洛素和硫黄醇的敏感性已注明.
- 分别在10.87%和28.26%的分离物中检测到tetA和blaTEM基因.
结论:
- 腹的羊羔有各种各样的大肠杆菌病变型,其中EAEC是最常见的.
- STEC和EPEC的存在凸显了潜在的风险.
- 广泛的抗菌素耐药性需要在兽医实践中谨慎使用抗生素.
- 检测到tetA和blaTEM基因表明,在绵羊大肠杆菌种群中存在抗生素耐药性的储备.
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