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转录组分析显示,因布苏病毒感染引起的热冲击蛋白70 (HSP70) 与免疫反应有关

D Zhao¹, K Han¹, L Zhang¹

¹Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing City, Jiangsu Province, 210014, PR China.

Polish journal of veterinary sciences

|December 30, 2024

概括

热冲击蛋白70 (HSP70) 通过抑制宿主,促进了Tembusu病毒 (TMUV) 感染.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

坦布苏病毒 (TMUV) 对水禽繁殖行业构成重大威胁.
TMUV感染的分子机制,特别是宿主免疫反应,仍然不太了解.
热冲击蛋白70 (HSP70) 被确定为TMUV感染中的积极调节者.

研究的目的:

在TMUV感染期间调查HSP70与宿主免疫反应之间的相互作用.
为了识别宿主基因在响应TMUV感染时有或没有HSP70抑制的不同表达.

主要方法:

用或没有HSP70抑制剂治疗的TMUV感染的DF-1细胞的深度测序.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
使用逆转录定量实时PCR验证选择的差异表达基因.

主要成果:

确定了43个差异表达基因 (DEG),其中39个在接受HSP70抑制剂治疗的细胞中显著下调.
DEGs主要参与天生的免疫反应途径,包括RIG-I类受体,通道类受体和NF-κB信号传递.
对12种与免疫相关的下调调节的DEG进行了转录组数据的验证.

结论:

HSP70在调节宿主对TMUV感染的天生的免疫反应方面发挥着至关重要的作用.
这项研究提供了关于HSP70在对TMUV.宿主反应中的作用的首次见解.
这些发现有助于理解TMUV病原体和宿主-病原体相互作用.

关键词:

热冲击蛋白质 70 70 热冲击蛋白质天生的免疫反应.坦布苏病毒 (Tembusu Virus) 是一种病毒.转录组分析转录组分析

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