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雅努斯 PEGylated CuS-engineered Lactobacillus casei通过代谢干扰和天生的免疫调节来对抗生物膜感染

Lingtong Kong¹, Xianli Hu², Demeng Xia³

¹Department of Orthopedics, The First Affiliated Hospital of Naval Medical University: Changhai Hospital, Shanghai, 200433, China.

|December 30, 2024

概括

工程益生菌通过使用纳米颗粒来产生有毒的基激素和温和的热量来对抗植入物感染,清除生物膜并刺激免疫反应以改善治疗结果.

科学领域:

生物材料工程生物材料工程
微生物学微生物学
免疫学免疫学免疫学

背景情况:

植入物相关的细菌感染往往会导致由于保护性生物膜导致假肢失败.
生物膜微环境的酸度和高水平的过氧化会抑制宿主免疫力,导致反复感染.

研究的目的:

开发一种工程益生菌 (LC@CuPen) 用于治疗与细菌植入物相关的感染.
调查LC@CuPen. 的抗菌膜疗效和免疫调节作用.

主要方法:

工程乳杆菌casei与詹姆斯PEGylatedCuS纳米颗粒 (CuPen) 创建LC@CuPen.
利用近红外 (NIR) 辐射激活CuPen,在生物膜内产生局部热和基 (•OH).
评估LC@CuPen对细菌新陈代谢,生物膜结构和巨细胞极化 (通过NF-κB通路进行M1极化) 的影响.

主要成果:

用NIR激活的LC@CuPen通过温和的热量和OH生产有效地破坏了L.casei和生物膜结构.
细菌素释放增强了OH透,导致细菌代谢中断和对热量的敏感性增加.
LC@CuPen诱导了M1巨细胞的两极分化,促进了炎症因子释放和细菌细胞,从而抑制了感染的复发.

结论:

工程L. casei (LC@CuPen) 为细菌植入物相关感染治疗提供了一种新的策略.
生物膜破坏和免疫刺激的双重作用方法对工程益生菌的临床应用有希望.

关键词:

细菌生物膜感染感染的细菌.在这种情况下,细菌素可以工程设计的益生菌.产生的免疫调节产生的免疫调节巨细胞是一个巨细胞.

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