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自然选择塑造了蚊子传播的致病性黄斑病毒中NS1的代使用和宿主适应

Ruichen Wang¹, Ziyi Li¹, Qikai Yin¹

¹National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

International journal of biological macromolecules

|December 30, 2024

概括

弗拉维病毒NS1蛋白质表现出由自然选择塑造的独特的编码子使用,有助于病毒适应和免疫逃避. 这种编码子偏差对于开发有效的NS1基疫苗来预防蚊子传播疾病至关重要.

科学领域:

分子生物学分子生物学
病毒学病毒学
进化生物学进化生物学

背景情况:

NS1蛋白质是蚊子传播的黄病毒的一个关键组成部分,涉及病毒复制,病变发生和免疫逃避.
了解NS1的进化动态和密码体使用模式对于开发有效的抗病毒策略和疫苗至关重要.

研究的目的:

为了研究九种不同的蚊子传播的黄斑病毒中NS1蛋白的编码子使用模式和进化特征.
阐明自然选择在塑造NS1编码子偏差中的作用及其对病毒适应和宿主免疫逃避的影响.

主要方法:

编码器使用的比较分析,包括相对同义编码器使用 (RSCU),有效编码器数量 (ENC) 和平价规则2 (PR2) 分析.
主要成分分析 (PCA) 用于评估代使用模式的相似性.
中立性和选择压力分析以确定塑造子偏差的力量.

主要成果:

NS1基因在不同类型的黄病毒中表现出明显的基组成偏差和非保守的密码子使用模式.
根据PCA,ENC和PR2分析显示,NS1基因的体使用模式在蚊子传播的黄病毒中保持不变.
自然选择,特别是净化选择,是NS1编码子使用的主要驱动因素,与其ORF相比,NS1中观察到的积极选择更强,增强宿主适应性和免疫逃避.

结论:

NS1蛋白的编码子使用模式是flavivirus适应和免疫逃避的一个重要因素.
鉴定到的NS1的进化特征和代码子偏差为合理设计和优化NS1基的针对蚊子传播的黄病毒疫苗提供了宝贵的见解.

关键词:

Codon 的使用模式主机适应性主机适应性蚊子传播的致病性黄病毒病毒.在NS1中,NS1是NS1.自然选择是自然选择.

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