在小鼠中EBF2升高,但不是猪,通过染色质激活驱动渐进的棕色脂肪系特征
Yinlong Liao1, Zhelun Peng2, Shanshan Fu3
1College of Animal Science, Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, Shandong Agricultural University, Taian, China; Yazhouwan National Laboratory, Sanya, China.
Journal of advanced research
|December 30, 2024
概括
早期B细胞因子2 (EBF2) 通过激活BAT基因驱动棕色脂肪 (BAT) 谱系的规范. 在猪中,低EBF2防止了BAT的形成,而不是在小鼠中,其升高促进了BAT的发展.
科学领域:
- 发育生物学是发展生物学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 进行比较的基因组学.
背景情况:
- 棕色脂肪组织 (BAT) 能够实现不发的热生成,但在像猪这样的物种中不存在.
- 最佳技术源源源于表达双盒7 (Pax7) 的中皮干细胞,这些干细胞也形成了骨肌肉.
- 控制棕色脂肪与肌肉血统决策的分子机制在物种之间尚不清楚.
研究的目的:
- 调查棕色脂肪和骨肌肉血统从Pax7+干细胞分离期间的表观遗传动力学.
- 为了确定重要的表观遗传因素,新的BAT形成.
- 了解在BAT开发中的物种特异性差异,特别是小鼠和猪之间的差异.
主要方法:
- 在谱系规范过程中分析染色体景观动态.
- 染色体免疫沉降测序 (ChIP-seq) 用于基因素修饰 (H3K4me3,H3K27me3,H3K27ac) 的测序.
- 关键转录因子的识别和功能分析,包括早期B细胞因子2 (EBF2).
主要成果:
- 在肌肉基因上有明显的组素标记的早期指定 (E12.5) 的肌源性祖先.
- 后来发生了BAT谱系规范 (E10.5-E14.5),在BAT基因上进行了渐进的组素修饰沉积.
- EBF2被确定为一个关键的驱动因素,与CBP/P300相互作用,通过H3K27ac.激活BAT基因.
- 在老鼠和猪的干细胞中,EBF2刺激了脂肪生成.
- 在关键窗口期间猪的EBF2表达的减少阻止了胚胎的BAT形成.
结论:
- EBF2是棕色脂肪生成的关键调节者,通过染色质激活促进谱系特征.
- 在EBF2表达时间的特定物种差异解释了与小鼠相比,猪中没有胚胎BAT.
- 了解EBF2的作用,可以了解BAT的进化损失和潜在的重新激活.
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