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Updated: Jul 27, 2026

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
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通过表面生物化检测等离子体膜蛋白的指导方针

Jae Won Roh1, Hye Won Choi2, Heon Yung Gee1

  • 1Department of Pharmacology, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722, Republic of Korea; Woo Choo Lee Institute for Precision Drug Development, Seoul 03722, Republic of Korea.

Molecules and cells
|December 30, 2024
PubMed
概括

我们开发了一种简单的生物化方法来分析血膜蛋白质. 这种技术使得这些关键的细胞表面蛋白质能够在最小的污染下精确地分离和分析.

关键词:
生物素是生物素中的一种.血膜中的等离子体膜.在Streptavidin中使用.表面生物化表面生物化

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相关概念视频

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.