托克索卡卡的第二阶段和第三阶段幼虫的超结构形态在形宿主组织内部
Letitia Amanda Theja1, Kusnoto Kusnoto2, Martia Rani Tacharina3
1Profession Program in Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Open veterinary journal
|December 31, 2024
概括
扫描电子显微镜揭示了Toxocara cati幼虫第二阶段 (L2) 和第三阶段 (L3) 之间的明显形态差异. 这些超结构性发现有助于诊断动物传播疾病 - - 毒素虫病.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 动物传播疾病 动物传播疾病
- 显微镜技术 显微镜技术
背景情况:
- 托克索卡卡提导致动物性疾病托克索卡里亚症.
- 寄生宿主对于传染Toxocara cati幼虫第二阶段 (L2) 是至关重要的.
- 扫描电子显微镜 (SEM) 提供了寄生虫幼虫阶段的详细3D成像.
研究的目的:
- 为了确定L2和L3的形态L2和L3Toxocara cati在paratenic宿主组织.
- 评估SEM在毒病的病因学诊断中的有用性.
主要方法:
- 作为寄生宿主的小鼠,接种了含有L2的胚胎T. cati蛋.
- 从小鼠和他们的后代收集L2和L3幼虫,经过特定的孕期和发育期.
- 使用SEM进行了超结构性检查,并对发现进行了描述性分析.
主要成果:
- SEM揭示了尺寸差异:L2幼虫比L3幼虫更小.
- 在L2和L3.3的中间和后部部分观察到明显的形态变异.
- 与L3相比,L2幼虫的身体环比L3更窄,更多,环间距离更大. L3的尾部尖端比L2.2有更多的曲线.
结论:
- SEM有效地区分了L2和L3的毒虫幼虫.
- 在中部和后部幼虫部分对身体环的超结构分析是区分阶段的关键.
- 基于SEM的形态表征有助于对毒素病的病因诊断.
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