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Porcine Model of Infrarenal Abdominal Aortic Aneurysm
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人体腹腔大动脉动脉瘤中CRP沉积与转录组变化与动脉瘤病变发生有关:来自in situ空间整体转录组分析的见解
Eun Na Kim1, Hee Young Seok2, Joon Seo Lim3
1Department of Pathology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
Frontiers in immunology
|December 31, 2024
概括
在腹腔大动脉动脉瘤 (AAA) 中的C反应性蛋白 (CRP) 沉积显著改变了巨细胞,淋巴细胞和光滑肌细胞中的基因表达. 这表明CRP促进炎症,亡和血管退化,有助于AAA的进展.
科学领域:
- 血管生物学 血管生物学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 腹腔大动脉动脉瘤 (AAA) 是一种危及生命的疾病,其特征是大动脉壁衰弱.
- C-反应蛋白 (CRP) 是一种炎症标记物,但其在AAA病原发生中的特定作用仍然不完全理解.
- 研究CRP对AAA壁内的基因表达的影响对于了解疾病机制至关重要.
研究的目的:
- 研究C反应性蛋白 (CRP) 沉积在腹腔大动脉动脉瘤 (AAA) 中血管壁上的影响.
- 分析AAA组织内基因表达的空间解析变化.
- 阐明导致AAA疾病进展的分子途径.
主要方法:
- 手术切除的AAA和正常的大动脉样本根据CRP水平和免疫组织化学 (IHC) 染色进行了分类.
- 在特定细胞群 (巨细胞,淋巴细胞,光滑肌肉细胞) 上进行了时空空间全转录组分析.
- 基因本体学 (GO) 分析用于识别差异表达的基因和相关途径.
主要成果:
- 在所有分析的细胞类型中,在高CRP和低CRPAAA组之间观察到显著的基因表达差异.
- 在巨细胞中,CRP与炎症,亡和免疫反应途径有关,涉及STAT3.
- 在光滑肌肉细胞中,CRP对NF-κB和JNK信号通路进行上调,同时对与血压调节和血管直径维持相关的基因进行下调.
结论:
- 在AAA中CRP沉积与关键血管和免疫细胞的实质性转录基因变化有关.
- 显然,CRP在AAA壁内促进了促炎和亡过程.
- 这些变化表明CRP有助于血管结构退化和弹性降低,可能导致AAA的进展.
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