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通过RNA-seq分析,在血清中发现了三种lncRNA小组,作为肌肉侵入性膀癌的潜在生物标志物

Xiumei Jiang¹, Ailin Qu¹, Shoucai Zhang¹

¹Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong, China.

Frontiers in oncology

|December 31, 2024

概括

在血清中,一个新的3长非编码RNA (lncRNA) 面板显示了肌肉侵入性膀癌 (MIBC) 早期诊断的前景. 该面板显示出高精度,可能有助于MIBC的非侵入性诊断策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物标志物发现发现

背景情况:

准确的手术前确定肌肉侵入性膀癌 (MIBC) 对于治疗规划至关重要.
在血清中需要早期诊断生物标志物来检测MIBC.

研究的目的:

探索基于血清的长非编码RNA (lncRNA) 生物标志物,用于MIBC的早期诊断.
开发一种诊断特征,以区分MIBC与非肌肉侵入性膀癌 (NMIBC).

主要方法:

使用高通量测序对lncRNA表达特征进行选.
在380名患者 (190名MIBC,190名NMIBC) 中通过RT-qPCR验证候选 lncRNA.
使用多变量逻辑回归和法根的名图的诊断签名的开发.

主要成果:

鉴定了三个差异表达的 lncRNAs:LINC00565,LINC00592 和 NDUFA6-AS1.1.
一个3-lncRNA小组实现了高的诊断准确性 (AUC 0.903培训,0.875验证).
费根的诺米图表明了实际的临床实用性;网络分析显示了LINC00565和特定的微RNA之间的相关性.

结论:

3-lncRNA血清面板对MIBC具有显著的诊断能力.
这些lncRNAs代表了早期MIBC检测的有希望的非侵入性生物标志物.
这些发现支持该面板在膀癌诊断中的潜在临床应用.

关键词:

生物标志物生物标志物诊断诊断诊断诊断诊断诊断在ncRNA中,我们可以肌肉侵入性膀癌是一种肌肉侵入性膀癌.血液中的血清是血清.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...