通过直接与OSLF促进体结合,OSPIL15诱导的米头日期延迟取决于功能性B细胞染色体

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¹Institute of Wetland Agriculture and Ecology, Shandong Academy of Agricultural Sciences, Jinan, China.

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概括

米标题日期对于作物产量至关重要. 过度表达的OsPIL15通过调节OsLF和与植物染色体B (PHYB) 相互作用来延迟开花,从而提供了对米发育的见解.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
农业科学农业科学

背景情况:

米标题日期是作物生产和适应性的关键决定因素.
了解控制开花时间的分子机制对于改善水种植至关重要.

研究的目的:

阐明OsPIL15延迟大米标志日期的分子机制.
确定OsPIL15的下游目标和参与开花时间调节的交互合作伙伴.

主要方法:

在大米 (Oryza sativa) 中过度表达和淘汰OsPIL15和OsLF基因.
染色体免疫沉降测序 (ChIP-seq) 和转录组测序 (RNA-seq) 试验.
在野生型和phyB突变大米中进行蛋白质-蛋白质相互作用测定和分析.

主要成果:

过度表达OsPIL15通过调节关键的开花时间基因 (Hd1,Hd3a,RFT1) 延迟了大米标题日期.
OsPIL15直接结合并激活OSLF的促进子,这是一个头部抑制器.
淘汰OsLF部分挽救了OsPIL15过度表达线的延迟开花表型.
OsPIL15的植物染色体B (PHYB) 结合 (APB) 基因对其与PHYB的相互作用至关重要,并且对标题日期的PHYB依赖的延迟.

结论:

由于OSLF的规定,OSPIL15部分推迟了大米标志日期.
由APB动机介导的OsPIL15和PHYB之间的相互作用对于在长日条件下观察到的航向日期延迟至关重要.

关键词:

美国的OSLF 活跃的植物染色体B结合动机.标题日期标题日期标题日期植物染色体B 植物染色体B 植物染色体与相互作用的因素.米米饭米饭米饭米饭.

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