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ALCAM是严重的社区获得性肺炎的入口因素,与肺炎相关的人类腺病毒B种类

Yusang Xie¹, Hong Mei², Wei Wang²

¹Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Institutes of Respiratory Diseases, School of Medicine, Shanghai Jiao Tong University and Shanghai Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases, Shanghai, China.

Nature communications

|December 31, 2024

概括

活性白细胞细胞粘附分子 (ALCAM) 被确定为人类腺病毒 B 种 (HAdV-B) 感染的新进入因素. 这一发现有助于我们更好地理解严重社区性肺炎 (SCAP) 的病原性.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
遗传学遗传学是一个

背景情况:

人类腺病毒 (HAdV) 引起广泛的感染,HAdV B 种 (HAdV-B) 与严重的社区获得性肺炎 (SCAP) 有关.
影响SCAP相关HAdV-B感染的宿主因素需要进行系统的调查.

研究的目的:

通过CRISPR查,识别参与HAdV-B感染的宿主因素.
调查确定的宿主因子在病毒入境中的作用及其与SCAP的关联.

主要方法:

进行了CRISPR基因查,使用细胞表面蛋白质专注的库来识别HAdV-B宿主因子.
进行了进一步的调查,包括病毒内化试验,患者血液样本的转录分析,以及仿真/真实病毒实验.
描述了HAdV-B纤维旋和确定的主体因子之间的结合亲和力.

主要成果:

确定了激活白细胞细胞粘附分子 (ALCAM) 作为HAdV-B感染的新型宿主因子,与已知的受体DSG-2一起.
通过参与病毒内部化,ALCAM促进了HAdV-B的进入.
与其他感染相比,患有HAdV-B感染的SCAP患者的ALCAM表达升高.
ALCAM 作为 HAdV-B 基因群 B1 和 B2 的宿主因子,HAdV-B 纤维旋结合的平均解离常数为 837 nM.

结论:

激活白细胞细胞粘附分子 (ALCAM) 是严重的社区获得性肺炎相关的人类腺病毒B种的重要进入因素.
这一发现为HAdV-B病原体和潜在的治疗点提供了新的见解.

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