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有氧化脂和转录的签名THC相关的vaping相关的肺损伤
Tomeka L Suber1, Mohammadreza Tabary2, William Bain2,3
1Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Pittsburgh, Pittsburgh, PA, USA. subertl2@upmc.edu.
Scientific reports
|December 31, 2024
概括
与THC产品相关的电子烟/vaping相关的肺损伤 (EVALI) 显示炎症介质增加和脂质配置文件发生变化. 支气管洗液的这些变化可能会导致EVALI患者的肺细胞损伤.
科学领域:
- 肺部医学 肺部医学
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
背景情况:
- 电子烟/吸烟相关的肺损伤 (EVALI) 经常与维生素E乙酸和四大麻素 (THC) 使用有关.
- 了解EVALI病原体背后的分子机制对于有效治疗和预防至关重要.
研究的目的:
- 在使用含有THC产品的患者中调查与EVALI相关的分子途径,包括细胞因子,转录和脂质形状.
- 探索来自EVALI患者的支气管膜洗液 (BALF) 对肺上皮细胞活性的影响.
主要方法:
- 从THC-EVALI的机械通风患者和使用Luminex多重测定,RNA测序和质谱的对照组分析BALF.
- 评估不同患者群体用BALF治疗的肺上皮细胞 (BEAS-2B) 释放的乳酸脱酶 (LDH).
主要成果:
- 在THC-EVALI BALF中,细胞因子 (IFNγ,CCL2,CXCL5) 和矩阵金属蛋白酶-2 (MMP2) 的水平升高.
- 转录组分析显示,生物氧化,葡萄糖化和脂肪酸代谢途径中的丰富.
- 在THC-EVALI BALF.中观察到油酸,酸代谢物和氧化酸乙醇胺 (PE),特别是PE的水平增加.
- 与对照组相比,THC-EVALI患者的BALF诱导了肺上皮细胞中显著更高的LDH释放.
结论:
- THC-EVALI的特点是具有独特的分子形状,涉及改变的脂成分和氧化脂质的积累.
- 在BALF中增加的促炎媒介和氧化脂可能有助于EVALI中的上皮细胞死亡.
- 这些发现为进一步研究氧化脂作为EVALI病变发生的关键参与者提供了基础.
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